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      產(chǎn)品詳情
      Chaetomium globosum Kunze : Fries拉丁名

      (ATCC? 6205?)菌株編號

      Strain Designations菌株別名 QM 459 [1042.4, CBS 148.51, CBS 161.52, CEB 1218.1, CEB 1218.2, CECT 2701, DSM 1962, IFO 6347, IMI 45550, MUCL 1984, NRRL 1870, UPSC 3159, VTT D-81079]

      Application Bacterial resistance testing adhesives Fungus resistance testing Fungus resistance testing adhesives

      Fungus resistance testing airborne equipment Fungus resistance testing aircraft transmissions Fungus resistance testing automotive components

      Fungus resistance testing packing materials Fungus resistance testing paper and paperboard Fungus resistance testing plastics

      Fungus resistance testing polymers Fungus resistance testing sandbags

      Fungus resistance testing textiles Produces endoglucanase Sterility assurance Testing aerosols Testing slimicide Degrades cotton fabric

      Biosafety Level 生物安全等級1

      Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

      Product Format提供形式 freeze-dried

      Storage Conditions Frozen保藏方法: -80℃ or colder

      Freeze-Dried凍干物: 2℃ to 8℃

      Live Culture活菌: See Propagation Section

      Type Strain模式菌株 no

      Preceptrol? no

      Genome Sequenced Strain Yes

      Comments Genome sequencing strain (Broad Institute, USA).

      Medium培養(yǎng)基 ATCC? Medium 329: Mineral salts agar

      ATCC? Medium 340: Rabbit food agar

      ATCC? Medium 307: Cornmeal agar

      Growth Conditions生長條件

      Temperature 培養(yǎng)溫度: 24℃ to 26℃

      Atmosphere需氧情況: Typical aerobic; Filter paper required as carbon source.

      Name of Depositor QM

      Chain of Custody來源國家  ATCC <-- QM <-- C Thom 1042.4

      Isolation分離源  Stored cotton, Washington, DC

      Cross References Nucleotide (GenBank) : KC146352 ITS including 5.8S rRNA gene

      Nucleotide (GenBank) : KC146368 D1/D2 region of 28S rRNA gene

      Nucleotide (GenBank) : AAFU00000000 Chaetomium globosum CBS 148.51, whole genome shotgun sequencing project

      Nucleotide (GenBank) : EF524036 Chaetomium globosum strain ATCC 6205 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence.

      References 參考文獻  Greathouse GA, Ames LM. Fabric deterioration by thirteen described and three new species of Chaetomium. Mycologia 37: 138-155, 1945.

      AATCC Antifungal Activity, Assessment on Textile Materials: Mildew and Rot Resistance of Textile Materials.

      Research Triangle Park, NC:American Association of Textile Chemists and Colorists;AATCC Test Method 30-2004.

      ASTM International Standard Test Method for Mildew (Fungus) Resistance of Paper and Paperboard.

      West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 2020-92 (Reapproved 2003).

      ASTM International Standard Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi.

      West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4300-01.

      ASTM International Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi.

      West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4783-01e1.

      ASTM International Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi.

      West Conshohocken, PA:ASTM International;ASTM Standard Test Method G 0021-96 (Reapproved 2002).

      Deutsches Institut fur Normung Testing of plastics: Influence of fungi and bacteria; visual evaluation, change in mass or physical properties.

      Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 53739. RTCA Environmental conditions and test procedures for airborne equipment -- Fungus resistance.

      Washington, DC:RTCA;RTCA DO-160C. U.S. Department of Defense Environmental test methods and engineering guidelines.

      Washington, DC:US Department of Defense;Military Standard MIL-STD-810F, 2000

      Bailey PJ, et al. Isolation and characterization of cellulase (beta-1,4-glucan 4-glucanohydrolase) from Chaetomium globosum Kunze. Mater. Org. 7: 27-43, 1972.

      Iannarone M, Blasecki JW Jr.. Microbiological testing of aerosol preparations using Chaetomium globosum. J. Pharm. Sci. 55: 1471-1472, 1966.

      Standard Test Method for Efficacy of Slimicides for the Paper Industry-Bacterial and Fungal Slime. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1839-07.

      Methods of testing materials for resistance to fungal growth. Part 2: Resistance of textiles to fungal growth.. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 1157.2-1998.

      Methods of testing materials for resistance to fungal growth. Part 3: Resistance of cordage and yarns to fungal growth. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 1157.3-1999.

      Methods of testing materials for resistance to fungal growth. Part 4: Resistance of coated fabrics and electronic boards to fungal growth. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 1157.4-1999.

      Plastics--Evaluation of the action of micro-organisms. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 846:1997.

      Sterilization of single-use medical devices incorporating materials of animal origin--Validation and routine control of sterilization by liquid chemical sterilants.

      Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14160:1998.

      Plastics--Assessment of the effectiveness of fungistatic compounds in plastics formulations.

      Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 16869:2001.

      Testing of textiles -- Evaluation of the action of microfungi. London, UK:British Standards Institution;British Standard BS EN 14119:2003, 2003 Standard Specification for Cellulosic Fiber Loose-Fill Thermal Insulation.

      West Conshohocken, PA:ASTM International;ASTM Standard Test Method C 0739-05be1. Standard Specification for Self-Supported Spray Applied Cellulosic Thermal Insulation.

      West Conshohocken, PA:ASTM International;ASTM Standard Test Method C 1149-06e1. Standard Test Method for Determining Fungi Resistance of Insulation Materials and Facings.

      West Conshohocken, PA:ASTM International;ASTM Standard Test Method C 1338-00. Standard Specification for Poly(Vinyl Chloride) (PVC) Plastic Flexible Concealed Water-Containment Membrane.

      West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4551-96 (Reapproved 2001).

      Plastics - Evaluation of the action of microorganisms. London, UK:British Standards Institution;British Standard BS EN ISO 846:1997.

      Sterilization of single-use medical devices incorporating materials of animal origin --- Validation and routine control of sterilization by liquid chemical sterilants, Annex A. London, UK:British Standards Institution;British Standard BS EN ISO 14160:1998.

      Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262

      Cuomo CA, et al. Draft genome sequence of the cellulolytic fungus Chaetomium globosum. Genome Announc. 3: e00021-15, 2015. PubMed: 25720678
      常見問題
      問:細胞的運輸方式有哪些?有什么區(qū)別?

      答:公司提供兩種運輸方式供老師選擇,1、復(fù)蘇的活細胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟,細胞成活率較高。2、凍存的細胞:采用干冰運輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲,擇機復(fù)蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點是需要自己復(fù)蘇。

      問:為什么你們的細胞和其他公司的細胞培養(yǎng)條件不一樣?

      答:我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進行選擇!

      問:培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎?

      答:不可以重復(fù)使用,一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多,通常在3-5%,以維持細胞存活,控制生長速度,不可以用來做細胞培養(yǎng)使用。

      問:培養(yǎng)細胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點是什么?

      答:細胞在鏡下發(fā)現(xiàn)圓形的白色的點點,一般情況下是為貼壁的細胞或脫落的細胞死亡后的產(chǎn)物,懸浮細胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點。通常,白色的圓點是分散分布的,聚團類的懸浮細胞可能會聚團出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

      問:剛買回來的細胞如何凍存留種呢?

      答:一般情況下,我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細胞數(shù)量多一些,便于后期復(fù)蘇。購買原代細胞的客戶,要充分考慮該細胞的傳代次數(shù)限制,人源原代細胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實驗。

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