LMG 19282
目錄價(jià)
￥ 0
一鍵復(fù)制產(chǎn)品信息
別稱(chēng)Cupriavidus Campinensis
貨號(hào)Delf-17318
規(guī)格
產(chǎn)品推薦
聯(lián)系方式
- 電話(huà)：400-1016-218
- 郵箱：355185756@qq.com
- 地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室
產(chǎn)品詳情
ATCC BAA-700 編號(hào)
Deposited As Ralstonia campinensis Goris et al.
Strain Designations 菌株別名 LMG 19282 [CCUG 44526, CIP 107177, WS2]
Isolation 分離源 Desert Belgium
Biosafety Level 安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Preceptrol no
Type Strain 模式菌株 yes Ref, Ref
Medium 培養(yǎng)基 ATCC Medium 18: Trypticase Soy Agar/Broth
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度 : 30.0°C
Atmosphere需氧情況 : Aerobic
Name of Depositor 寄存人 BCCM/LMG Laboratory of Microbiology
Special Collection NSF - Bacteriology
Chain of Custody
BCCM/LMG Laboratory of Microbiology
M Mergeay
Isolation 分離源
Desert
Belgium
References 參考文獻(xiàn)
Goris J, et al. Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al.. Int. J. Syst. Evol. Microbiol. 51: 1773-1782, 2001. PubMed: 11594608
zinc desert, Lommel
Vaneechoutte M, et al. Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov.. Int. J. Syst. Evol. Microbiol. 54: 317-327, 2004. PubMed: 15023939
Vandamme P, Coenye T. Taxonomy of the genus Cupriavidus: a tale of lost and found. Int. J. Syst. Evol. Microbiol. 54: 2285-2289, 2004. PubMed: 15545472
Goris J, et al. Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al.. Int. J. Syst. Evol. Microbiol. 51: 1773-1782, 2001. PubMed: 11594608
Vaneechoutte M, et al. Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov.. Int. J. Syst. Evol. Microbiol. 54: 317-327, 2004. PubMed: 15023939
Cross References
Nucleotide (GenBank) : AF312020 Ralstonia campinensis strain WS2 16S ribosomal RNA gene, partial sequence.
常見(jiàn)問(wèn)題
答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過(guò)夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。
答：我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶(hù)提供了替代培養(yǎng)方案，根據(jù)客戶(hù)的意愿進(jìn)行選擇！
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來(lái)做細(xì)胞培養(yǎng)使用。
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類(lèi)的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶(hù)收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買(mǎi)原代細(xì)胞的客戶(hù)，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。
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