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產品詳情
W1219
Other collection no.
or WDCM no.:
ATCC BAA-170,CIP 108362
Isolated from:
periodontal pocket
Country:
United Kingdom of Great Britain and Northern Ireland
Cardiff
Date of sampling:
before 22.12.1999
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- J. Downes; W1219 <- W. Wade; N/A (Eubacterium sp.)
Cultivation conditions:
Medium 104 (add 0.5 g/l Tween 80), anaerobic, 35°C
or
Medium 110 (add 0.5 g/l Tween 80), anaerobic, 35°C
Medium/media recipe(s) required for preparing 110:
- Medium 78
Please follow special instructions: 'Cultivation of Anaerobes'
Complete DSMZ Media List
Summary and
additional information:
<- J. Downes; W1219 <- W. Wade; N/A (Eubacterium sp.). Periodontal pocket; United Kingdom, Cardiff (7747). Type strain. Taxonomy/description (7747). (Medium 104 (add 0.5 g/l Tween 80), 35°C, anaerobic or Medium 110 (add 0.5 g/l Tween 80), 35°C, anaerobic).
Literature:
7747
Risk group:
2 (classification according to German TRBA)
Restrictions:
Act dealing with the prevention & control of infectious diseases in man (Infektionsschutzgesetz), Category A1
Supplied as:
Delivery formPrices
Freeze Dried
Active culture on request
DNA
Price Category for this culture: 1
Freight and handling charges will be added. See price list.
Note: Freight charges for Risk Group 2 microorganisms will apply.
Other collection no.
or WDCM no.:
ATCC BAA-170,CIP 108362
Isolated from:
periodontal pocket
Country:
United Kingdom of Great Britain and Northern Ireland
Cardiff
Date of sampling:
before 22.12.1999
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- J. Downes; W1219 <- W. Wade; N/A (Eubacterium sp.)
Cultivation conditions:
Medium 104 (add 0.5 g/l Tween 80), anaerobic, 35°C
or
Medium 110 (add 0.5 g/l Tween 80), anaerobic, 35°C
Medium/media recipe(s) required for preparing 110:
- Medium 78
Please follow special instructions: 'Cultivation of Anaerobes'
Complete DSMZ Media List
Summary and
additional information:
<- J. Downes; W1219 <- W. Wade; N/A (Eubacterium sp.). Periodontal pocket; United Kingdom, Cardiff (7747). Type strain. Taxonomy/description (7747). (Medium 104 (add 0.5 g/l Tween 80), 35°C, anaerobic or Medium 110 (add 0.5 g/l Tween 80), 35°C, anaerobic).
Literature:
7747
Risk group:
2 (classification according to German TRBA)
Restrictions:
Act dealing with the prevention & control of infectious diseases in man (Infektionsschutzgesetz), Category A1
Supplied as:
Delivery formPrices
Freeze Dried
Active culture on request
DNA
Price Category for this culture: 1
Freight and handling charges will be added. See price list.
Note: Freight charges for Risk Group 2 microorganisms will apply.
常見問題
問：細胞的運輸方式有哪些？有什么區(qū)別？
答：公司提供兩種運輸方式供老師選擇，1、復蘇的活細胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復蘇的步驟，細胞成活率較高。2、凍存的細胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉入液氮長期存儲，擇機復蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復蘇。
問：為什么你們的細胞和其他公司的細胞培養(yǎng)條件不一樣？
答：我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案，根據客戶的意愿進行選擇！
問：培養(yǎng)瓶的培養(yǎng)液可以重復使用嗎？
答：不可以重復使用，一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細胞存活，控制生長速度，不可以用來做細胞培養(yǎng)使用。
問：培養(yǎng)細胞在鏡下觀察發(fā)現有一些白色的圓點是什么？
答：細胞在鏡下發(fā)現圓形的白色的點點，一般情況下是為貼壁的細胞或脫落的細胞死亡后的產物，懸浮細胞也會有這種現象，出現圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團類的懸浮細胞可能會聚團出現白色的亮斑，技術老師可以繼續(xù)培養(yǎng)并觀察。
問：剛買回來的細胞如何凍存留種呢？
答：一般情況下，我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細胞數量多一些，便于后期復蘇。購買原代細胞的客戶，要充分考慮該細胞的傳代次數限制，人源原代細胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數很少的原代細胞，不建議凍存，收到后調整狀態(tài)后即可安排實驗。
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