Alkalicoccus Urumqiensis
目錄價(jià)
¥ 0
一鍵復(fù)制產(chǎn)品信息
別稱Alkalicoccus Urumqiensis
貨號(hào)Delf-17450
規(guī)格
產(chǎn)品推薦
聯(lián)系方式
- 電話:400-1016-218
- 郵箱:355185756@qq.com
- 地址:安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室
產(chǎn)品詳情
Taxonomy in NCBI database: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae.
30195T <-- B. Zhao; Grad. Sch. of Chin. Acad. of Agric. Sci., China; BZ-SZ-XJ18.
Accessioned in 2014.
=DSM 29145.
Bacillus urumqiensis.
Type strain [11131,13168].
Medium: 1046; Temperature: 35°C; Rehydration fluid: 656.
Source: Mixed water and sediment of a saline-alkaline lake, the Xinjiang Uyghur Autonomous Region, China [11131].
Biochemistry/Physiology: [11131].
Cell wall: Type A1γ (meso-A2pm) [11131].
Fatty acid: a-C15:0, n-C16:0, i-C15:0, a-C17:0, i-C16:0 [11131].
Quinone: MK-7 [11131].
Polar lipid: DPG, PG [11131].
G+C (mol%): 42.3 (HPLC), 41.4 (Tm) [11131].
DNA-DNA relatedness: [11131].
Phylogeny: 16S rRNA gene (KM066107) [11131]; Concatenated sequences for 650 core proteins [13168].
Other taxonomic data: Conserved signature indels [13168].
Genome sequence: PVNS00000000.
NCBI Taxonomy ID: 1548213.
Publication(s) using this strain [C16252].
Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information -
Additional information -
(1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
(2) in complying with the Plant Protection Law of Japan
Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request) information
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
常見問題
答:公司提供兩種運(yùn)輸方式供老師選擇,1、復(fù)蘇的活細(xì)胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟,細(xì)胞成活率較高。2、凍存的細(xì)胞:采用干冰運(yùn)輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ),擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快,一般一兩天即可收到,缺點(diǎn)是需要自己復(fù)蘇。
答:我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進(jìn)行選擇!
答:不可以重復(fù)使用,一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多,通常在3-5%,以維持細(xì)胞存活,控制生長(zhǎng)速度,不可以用來做細(xì)胞培養(yǎng)使用。
答:細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn),一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物,懸浮細(xì)胞也會(huì)有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常,白色的圓點(diǎn)是分散分布的,聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答:一般情況下,我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細(xì)胞數(shù)量多一些,便于后期復(fù)蘇。購(gòu)買原代細(xì)胞的客戶,要充分考慮該細(xì)胞的傳代次數(shù)限制,人源原代細(xì)胞大概可以傳7代左右,鼠源的可以傳3代左右,對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。









皖公網(wǎng)安備 34010402703761號(hào)
郭經(jīng)理:3554285629






