Mucilaginibacter Inviolabilis
目錄價
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一鍵復制產(chǎn)品信息
別稱Mucilaginibacter Inviolabilis
貨號Delf-17490
規(guī)格
產(chǎn)品推薦
聯(lián)系方式
- 電話:400-1016-218
- 郵箱:355185756@qq.com
- 地址:安徽省合肥市高新區(qū)黃山路602號合肥國家大學科技園A401室
產(chǎn)品詳情
Taxonomy in NCBI database: Bacteria; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae.
34116T <-- S.-R. Ko; KRIBB, South Korea; HC2.
Accessioned in 2020.
=KCTC 82084.
Type strain [13321].
Medium: 346; Temperature: 25°C; Rehydration fluid: 663.
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Source: Phycosphere of Haematococcus lacustris NIES 144 culture [13321].
Biochemistry/Physiology: [13221].
Fatty acid: [13221].
Quinone: MK-7 [13221].
Polar lipid: [13221].
G+C (mol%): 42.0 (genome sequence) [13221].
Phylogeny: 16S rRNA gene (MT197490) [13221].
Other taxonomic data: Average nucleotide identity, genome-to-genome distances [13321].
Genome sequence: JAANAT000000000 [13221].
NCBI Taxonomy ID: 2714892.
Related information on delivery / use of the strain
Biosafety level1
Terms and conditionsNot applicable
Export control (1)No
Distribution control in Japan (2)No
Genetically modified microorganismNo
Technical information-
Additional information-
(1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
(2) in complying with the Plant Protection Law of Japan
Delivery category
DomesticA (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
OverseasA (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
常見問題
答:公司提供兩種運輸方式供老師選擇,1、復蘇的活細胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復蘇的步驟,細胞成活率較高。2、凍存的細胞:采用干冰運輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲,擇機復蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點是需要自己復蘇。
答:我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進行選擇!
答:不可以重復使用,一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多,通常在3-5%,以維持細胞存活,控制生長速度,不可以用來做細胞培養(yǎng)使用。
答:細胞在鏡下發(fā)現(xiàn)圓形的白色的點點,一般情況下是為貼壁的細胞或脫落的細胞死亡后的產(chǎn)物,懸浮細胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點。通常,白色的圓點是分散分布的,聚團類的懸浮細胞可能會聚團出現(xiàn)白色的亮斑,技術老師可以繼續(xù)培養(yǎng)并觀察。
答:一般情況下,我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細胞數(shù)量多一些,便于后期復蘇。購買原代細胞的客戶,要充分考慮該細胞的傳代次數(shù)限制,人源原代細胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實驗。









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