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產品詳情
Deposit type:Bacteria
Type strain:Yes
GMO:No
Taxon name:Tissierella praeacuta
Depositor designation:Prevot 3722B
Preservation method:Lyophilised
Other catalogue information :
Former NCFB organism
Price band :B
Price(?):239
Media :389
Gas regime:anaerobic
Gas mixture:CO2 Hungate
Incubation temperature (and/or range):37°C
ACDP category :1
Colony Edge:Entire
Colony Size:1mm
Colony Surface:Smooth/Shiny
Colony Elevation:Low Convex
Colony Colour:Off White
Colony Opacity:Translucent
Cellular Shape:Rod
Cellular Size:medium
Cellular Arrangement:Single/Pairs
Gram stain:Gram Negative
Cellular (Other):Parallel/Rounded
Depositor Company :National Collection of Type Cultures (NCTC)
Depositor Address:61 Colindale Avenue London U.K. NW9 5HT
Depositor Telephone:+44 81 2004400
Source :gangrene
Date of Accession:1994-01-01
Other names:Tisserella praecuta
Other collection IDs:ATCC25539 NCFB3038 NCTC11158 VPI0563 DSM18095
Yeast?:False
K12:No
References:
IJSEM (2004) 54:947 Approved Lists of Bacterial Names, Int J Syst Bacteriol 30, 225-420, 1980 Multilocus analysis reveals diversity in the genus Tissierella: description of Tissierella carlieri sp. nov. in the new class Tissierellia classis nov, Syst Appl Microbiol 37(1), 23-34, 2014 Phylogenetic evidence that the gram-negative nonsporulating bacterium Tissierella (Bacteroides) praeacuta is a member of the Clostridium subphylum of the gram-positive bacteria and description of Tissierella creatinini sp. nov, Int J Syst Bacteriol 45(3), 436-440, 1995 Proposal of neotype strains for seven non-saccharolytic Bacteroides species, Int J Syst Bacteriol 29, 427-434, 1979 Reclassification of Bacteroides praeacutus Tissier (Holdeman an Moore) in a new genus, Tissierella, as Tissierella praeacuta comb. nov, Int J Syst Bacteriol 36, 461-463, 1986
Type strain:Yes
GMO:No
Taxon name:Tissierella praeacuta
Depositor designation:Prevot 3722B
Preservation method:Lyophilised
Other catalogue information :
Former NCFB organism
Price band :B
Price(?):239
Media :389
Gas regime:anaerobic
Gas mixture:CO2 Hungate
Incubation temperature (and/or range):37°C
ACDP category :1
Colony Edge:Entire
Colony Size:1mm
Colony Surface:Smooth/Shiny
Colony Elevation:Low Convex
Colony Colour:Off White
Colony Opacity:Translucent
Cellular Shape:Rod
Cellular Size:medium
Cellular Arrangement:Single/Pairs
Gram stain:Gram Negative
Cellular (Other):Parallel/Rounded
Depositor Company :National Collection of Type Cultures (NCTC)
Depositor Address:61 Colindale Avenue London U.K. NW9 5HT
Depositor Telephone:+44 81 2004400
Source :gangrene
Date of Accession:1994-01-01
Other names:Tisserella praecuta
Other collection IDs:ATCC25539 NCFB3038 NCTC11158 VPI0563 DSM18095
Yeast?:False
K12:No
References:
IJSEM (2004) 54:947 Approved Lists of Bacterial Names, Int J Syst Bacteriol 30, 225-420, 1980 Multilocus analysis reveals diversity in the genus Tissierella: description of Tissierella carlieri sp. nov. in the new class Tissierellia classis nov, Syst Appl Microbiol 37(1), 23-34, 2014 Phylogenetic evidence that the gram-negative nonsporulating bacterium Tissierella (Bacteroides) praeacuta is a member of the Clostridium subphylum of the gram-positive bacteria and description of Tissierella creatinini sp. nov, Int J Syst Bacteriol 45(3), 436-440, 1995 Proposal of neotype strains for seven non-saccharolytic Bacteroides species, Int J Syst Bacteriol 29, 427-434, 1979 Reclassification of Bacteroides praeacutus Tissier (Holdeman an Moore) in a new genus, Tissierella, as Tissierella praeacuta comb. nov, Int J Syst Bacteriol 36, 461-463, 1986
常見問題
問：細胞的運輸方式有哪些？有什么區(qū)別？
答：公司提供兩種運輸方式供老師選擇，1、復蘇的活細胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復蘇的步驟，細胞成活率較高。2、凍存的細胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉入液氮長期存儲，擇機復蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復蘇。
問：為什么你們的細胞和其他公司的細胞培養(yǎng)條件不一樣？
答：我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進行選擇！
問：培養(yǎng)瓶的培養(yǎng)液可以重復使用嗎？
答：不可以重復使用，一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細胞存活，控制生長速度，不可以用來做細胞培養(yǎng)使用。
問：培養(yǎng)細胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點是什么？
答：細胞在鏡下發(fā)現(xiàn)圓形的白色的點點，一般情況下是為貼壁的細胞或脫落的細胞死亡后的產物，懸浮細胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團類的懸浮細胞可能會聚團出現(xiàn)白色的亮斑，技術老師可以繼續(xù)培養(yǎng)并觀察。
問：剛買回來的細胞如何凍存留種呢？
答：一般情況下，我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細胞數(shù)量多一些，便于后期復蘇。購買原代細胞的客戶，要充分考慮該細胞的傳代次數(shù)限制，人源原代細胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細胞，不建議凍存，收到后調整狀態(tài)后即可安排實驗。
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