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      文獻(xiàn)征集
      產(chǎn)品詳情
      General information
      NCIMB number
      NCIMB 10664
      Deposit type
      Bacteria
      Type strain
      Yes
      Nagoya restrictions
      No
      GMO
      No
      Taxon name
      Clostridium haemolyticum
      Depositor designation
      McClung2027 Plummer29866
      Preservation method
      Lyophilised
      Price band
      A
      Price(£)
      130
      Media
      012
      Gas regime
      anaerobic
      Incubation temperature (and/or range)
      37°C
      ACDP category
      2
      Hazard information
      This species has been associated with pathogenic conditions in cattle, sheep and laboratory animals. Produced phospholipase C haemolyses red blood cells.
      Additional hazard information
      Clostridium haemolyticum is a recognized animal pathogen. It produces a variety of toxins including a phospholipase which causes a fatal haemolysis in cattle.
      Cellular Shape
      Rod
      Cellular Motility
      Yes
      Gram stain
      Gram Positive
      Depositor Name
      G.Hobbs
      Depositor Company
      Torry Research Station
      Depositor Address
      Aberdeen U.K.
      Source
      Bovine
      Date of Accession
      01/09/1971
      Other collection IDs
      ATCC9650 DSM5565
      Yeast?
      False
      K12
      No
      References
      Approved Lists of Bacterial Names, Int J Syst Bacteriol 30, 225-420, 1980 Phylogenetic basis for a taxonomic dissection of the genus Clostridium, FEMS Immunol Med Microbiol 24(3), 253-258, 1999 Phylogenetic positions of Clostridium novyi and Clostridium haemolyticum based on 16S rDNA sequences, Int J Syst Evol Microbiol 51(3), 901-904, 2001 Development and validation of a multiplex real-time PCR for detection of Clostridium chauvoei and Clostridium septicum, Mol Cell Probes 24(4), 204-210, 2010 Rapid identification and differentiation of pathogenic clostridia in gas gangrene by polymerase chain reaction based on the 16S-23S rDNA spacer region, Res Vet Sci 69(3), 289-294, 2000 Taxonomy of the Clostridia: ribosomal ribonucleic acid homologies among the species, J Gen Microbiol 88(2), 229-244, 1975 Phylogenetic analysis and PCR detection of Clostridium chauvoei, Clostridium haemolyticum, Clostridium novyi types A and B, and Clostridium septicum based on the flagellin gene, Vet Microbiol 86(3), 257-267, 2002
      常見問題
      問:細(xì)胞的運(yùn)輸方式有哪些?有什么區(qū)別?

      答:公司提供兩種運(yùn)輸方式供老師選擇,1、復(fù)蘇的活細(xì)胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟,細(xì)胞成活率較高。2、凍存的細(xì)胞:采用干冰運(yùn)輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲,擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點(diǎn)是需要自己復(fù)蘇。

      問:為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣?

      答:我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進(jìn)行選擇!

      問:培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎?

      答:不可以重復(fù)使用,一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多,通常在3-5%,以維持細(xì)胞存活,控制生長速度,不可以用來做細(xì)胞培養(yǎng)使用。

      問:培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么?

      答:細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn),一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物,懸浮細(xì)胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常,白色的圓點(diǎn)是分散分布的,聚團(tuán)類的懸浮細(xì)胞可能會聚團(tuán)出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

      問:剛買回來的細(xì)胞如何凍存留種呢?

      答:一般情況下,我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細(xì)胞數(shù)量多一些,便于后期復(fù)蘇。購買原代細(xì)胞的客戶,要充分考慮該細(xì)胞的傳代次數(shù)限制,人源原代細(xì)胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細(xì)胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。

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