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ATCC 10864 黑曲霉

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貨號(hào)Delf-18101

規(guī)格 凍干粉/培養(yǎng)物

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聯(lián)系方式

電話：
400-1016-218
郵箱：
355185756@qq.com
地址：
安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室

產(chǎn)品詳情

Organism:	Aspergillus niger van Tieghem, anamorph
Designations:	NRRL 330 [4050, CBS 122.49, CECT 2775, IFO 6661, IMI 60286, MUCL 28817, WB 330]
Isolation:	Chinese galls
Depositors:	NRRL
History:	ATCC <<--NRRL<<--C. Thom 4050 <<--- Eastman Kodak Co.
Biosafety Level:	1
Shipped:	freeze-dried
Growth Conditions:	ATCC medium 325: Malt extract agar （Blakeslee's formula） Temperature: 24.0°C
ATCC 10864 黑曲霉
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:	degrades unhydrolyzed potato starch [860] produces acetyl-xylan esterase [58340] produces acetylesterase acetyl esterase [18117] produces alpha-glucosidase maltase produces amylase, alpha 1A; salivary Amylase, salivary, alpha-1A produces ethyl alcohol ethanol [860] produces glucoamylase [46568] produces monoamine oxidase [17694] produces saccharifying enzymes [52819] transformation host [13801] [15113] [16756] produces large amounts of maltase and smaller amounts of alpha-amylase [49287] produces ethanol from potato starch when cocultured with Saccharomyces cerevisiae ATCC 26603 [860]
Subcollection:	Fungi
References:	860: Abouzied MM, Reddy CA. Direct fermentation of potato starch to ethanol by cocultures of Aspergillus niger and Saccharomyces cerevisiae. Appl. Environ. Microbiol. 52: 1055-1059, 1986. PubMed: 3539016 13801: Mohr G, Esser K. Improved transformation frequency and heterologous promoter recognition in Aspergillus niger. Appl. Microbiol. Biotechnol. 34: 63-70, 1990. PubMed: 1366974 15113: Brandhorst T, Kenealy WR. Effects of leader sequences upon the heterologous expression of restrictocin in Aspergillus nidulans and Aspergillus niger. Can. J. Microbiol. 41: 601-611, 1995. PubMed: 7641142 16756: . . DECHEMA Biotechnol. Conf. 3A: 319-323, 1989. 17694: Hoover LK, et al. Biotransformation of dopamine to norlaudanosoline by Aspergillus niger. Biotechnol. Bioeng. 38: 1029-1033, 1991. 18117: Linden J, et al. Purification and characterization of an acetyl esterase from Aspergillus niger. Appl. Biochem. Biotechnol. 45-46: 383-393, 1994. PubMed: 8010767 46568: Pazur JH, et al. Glycoenzymes: Structure and properties of the two forms of glucoamylase from Aspergillus niger. Carbohydr. Res. 20: 83-96, 1971. PubMed: 5151200 49287: Dingle J, Solomons GL. Enzymes from micro-fungi. J. Appl. Chem. 2: 395-399, 1952. 52819: Corman J, Langlykke AF. Action of mold enzymes in starch saccharification. Cereal Chem. 25: 190-201, 1948. 58340: Khan AW, et al. Comparison of natural hemicellulose and chemically acetylated xylan as substrates for the determination of acetyl-xylan esterase activity in Aspergilli. Enzyme Microb. Technol. 12: 127-131, 1990.

常見問題

問：細(xì)胞的運(yùn)輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。

問：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！

問：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來做細(xì)胞培養(yǎng)使用。

問：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問：剛買回來的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。