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菌 種 說 明
您好：
感謝您使用本單位的微生物菌種，我們有可用于科研、教學(xué)、生產(chǎn)的菌種約2500株，基本覆蓋了環(huán)境、工業(yè)、農(nóng)業(yè)、分析、食、藥用真菌行業(yè)各類生產(chǎn)和科研用微生物，其中許多優(yōu)良菌株的生產(chǎn)性能具有較高水平。我們對所提供菌種作如下說明：
菌種簡介：
1、菌種名稱：ATCC 9027 銅綠假單胞菌(綠膿桿菌)
2、規(guī)格：斜面□ 凍干管□
二、保藏條件：凍干菌種應(yīng)在2-8℃保存；斜面菌種放15-20度保存，15-20天轉(zhuǎn)接一次。
三、培養(yǎng)條件：
1. 培養(yǎng)基：營養(yǎng)瓊脂培養(yǎng)基（NA）
胰蛋白棟大豆肉湯培養(yǎng)基（TSA）
LB培養(yǎng)基 以上培養(yǎng)基三選一即可
2. 培養(yǎng)溫度： 37℃
3. 培養(yǎng)時(shí)間：18- 24h
四、真空凍干菌種的復(fù)蘇培養(yǎng)：
1、安瓿管開封：用浸過75%酒精的脫脂棉擦凈安瓿管，用火焰加熱其頂端，滴少量無菌水至加熱頂端使之破裂，用銼刀或鑷子敲下已破裂的安瓿管頂端。
2、菌種恢復(fù)培養(yǎng)：用無菌吸管吸取 0.1-0.2毫升的培養(yǎng)液或無菌水，滴入安瓿管內(nèi)輕輕振蕩，使凍干菌體溶解呈懸浮狀，吸取全部菌體懸浮液，移植于1-2支建議的斜面培養(yǎng)基上，并在建議的溫度下培養(yǎng)。
五、注意事項(xiàng)：
1、微生物菌種應(yīng)保藏于低溫、清潔和干燥的地方，室溫放置時(shí)間過長會(huì)導(dǎo)致菌種衰退；
2、菌種操作應(yīng)在無菌條件下進(jìn)行，防止雜菌污染；
3、凍干菌復(fù)蘇后，應(yīng)根據(jù)菌種狀況及時(shí)轉(zhuǎn)接傳代
Organism:Pseudomonas aeruginosa （Schroeter） MigulaDesignations:R. Hugh 813Isolation:outer ear infectionDepositor:CP HegartyBiosafety Level:2Shipped:freeze-driedGrowth Conditions:ATCC medium3: Nutrient agar or nutrient brothTemperature: 37.0°CPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Applications:assay of [4101] assay of antimicrobial preservatives [11020] [21514] [21603] detection of [92787] efficacy testing [92779] media testing [11018] [11019] [21511] [92429] preparatory test control quality control strain [92096] [92409] sterility testing [21604] [92306] produces rhamnolipid surfactant [9924]Related Products:purified DNA: ATCC 9027D-5ATCC 9027 銅綠假單孢菌References:4101: ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA9924: Zhang Y, Miller RM. Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant （biosurfactant）. Appl. Environ. Microbiol. 58: 3276-3282, 1992. PubMed: 144436311018: British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 200311019: British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 200311020: British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI C, 200321511: European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility （tests for specified micro-organisms）. Nutritive and selective properties of the media and validity of the test for specified micro-organisms. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.13, 199721514: European Pharmacopoeia Commission Efficacy of antimicrobial preservation. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.3, 199721603: U.S. Pharmacopeia General Chapters:ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia;USP USP28-NF23, 200521604: U.S. Pharmacopeia General Chapters:STERILITY TESTS. Rockville, MD:U.S. Pharmacopeia;USP USP28-NF23, 200532185: Jones CB, Platt JH. Propofol composition containing edetate. US Patent 5,714,520 dated Feb 3 199892096: Applied Biosystems MicroSeq Pharmaceutical Panel #1. :Applied Biosystems;MicroSeq92287: Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10.92304: Microbial limit test. Tokyo, Japan:Japanese Pharmacopoeia;JP Jp14e.part I.35.92306: Sterility test, Growth promotion test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.54.92307: Preservatives-effectiveness test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.12.92402: Ophthalmic optics--Contact lens care products--Microbiological requirements and test methods for products and regimens for hygienic management of contact lenses. Geneva （Switzerland）:International Organization for Standardization/ANSI;ISO ISO 14729:2001.92403: Ophthalmic optics--Contact lens care products--Antimicrobial preservative efficacy testing and guidance on determining discard date. Geneva （Switzerland）:International Organization for Standardization/ANSI;ISO ISO 14730:2000.92409: Applied Biosystems MicroSeq PHL Panel #1. :Applied Biosystems;MicroSeq92429: Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 200392779: Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria. London, UK:British Standards Institution;British Standard BS ISO 21149:2006.92787: Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa. London, UK:British Standards Institution;British Standard BS ISO 22717:2006.
常見問題
答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲，擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。
答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。
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