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Preceptrol? CultureOrganism:Burkholderia cepacia （Palleroni and Holmes） Yabuuchi et al. deposited as Pseudomonas cepacia （Burkholder） Palleroni and HolmesDesignations:UCB 717 [717-ICPB 25, Ballard 717, CCUG 12691, CCUG 13226, CFBP 2227, CIP 80.24, DSM 7288, HAMBI 1976, ICMP 5796, IFO 14074, JCM 5964, LMG 1222, NCCB 76047, NCPPB 2993, NCTC 10743, NRRL B-14810]Isolation:plant-derived foodstuff （onion, Allium cepa）Isolation date: 1948Depositor:RW Ballard, WH BurkholderBiosafety Level:2 ATCC 25416 蔥頭伯克氏菌Shipped:freeze-driedGrowth Conditions:ATCC medium3: Nutrient agar or nutrient brothTemperature: 30.0°C Duration: aerobicPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Cross References:Nucleotide （GenBank） : M22518P. cepacia 16S ribosomal RNA, complete cds.Nucleotide （GenBank） : AJ293344Burkholderia cepacia partial alkB gene for putative alkane 1-monooxygenase.Nucleotide （GenBank） : U85788Burkholderia cepacia plasmid pPEC320 polygalacturonase （pehA） gene, complete cds.Nucleotide （GenBank） : L36602Rhizobium meliloti （clone Rhizo70-1） heat shock protein 70 （hsp70） gene, complete cds. Nucleotide （GenBank） : L36603 Pseudomonas cepacia （clone Psudom70-1） heat shock protein 70 （hsp70） gene, complete cds.Nucleotide （GenBank） : U51235Mycoplasma capricolum ClpB （clpB）, partial cds, GrpE （grpE）, and DnaK （dnaK） genes, complete cds, and DnaJ （dnaJ） gene, partial cds.Nucleotide （GenBank） : U96927Burkholderia cepacia 16S ribosomal RNA gene, partial sequence.Type Strain:yes [8533] [9051] [10872]Comments:This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified. taxonomy [7673] Pyrimidine synthesis [10155]Applications:assay of [92287] assay of antimicrobial preservatives [4101] media testing [21529] produces cepabactin [1834] quality control strain [92096] [92409]Related Products:also available as SafeTsource?:ATCC 25416NAReferences:1472: Meyer JM, et al. Cepabactin from Pseudomonas cepacia, a new type of siderophore. J. Gen. Microbiol. 135: 1479-1487, 1989. PubMed:25332441834: Gluzman Y, Ahrens B. SV40 early mutants that are defective for viral DNA synthesis but competent for transformation of cultured rat and simian cells. Virology 123: 78-92, 1982. PubMed: 62931954101: ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA7673: Ballard RW, et al. Taxonomy of the aerobic pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola and P. caryophylli. J. Gen. Microbiol. 60: 199-214, 1970. PubMed: 54880548533: Palleroni NJ, Holmes B. Pseudomonas cepacia sp. nov., nom. rev.. Int. J. Syst. Bacteriol. 31: 479-481, 1981.9051: . Validation list no. 45. Int. J. Syst. Bacteriol. 43: 398-399, 1993.10155: Li K, West TP. Pyrimidine synthesis in Burkholderia cepacia ATCC 25416. Lett. Appl. Microbiol. 21: 340-343, 1995. PubMed: 757653010872: Yabuuchi E, et al. Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia （Palleroni and Holmes 1981） comb. nov. [published erratum appears in Microbiol Immunol 37: 335, 1995]. Microbiol. Immunol. 36: 1251-1275, 1992. PubMed: 128377421529: CLSI Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA:Clinical and Laboratory Standards Institute;CLSI M22-A3.28164: Hubner A, Hendrickson W. A fusion promoter created by a new insertion sequence, IS1490, aciivates transcription of 2,4,5-trichlorophenoxyacetic acid catabolic genes in Burkholderia cepacia AC1100. J. Bacteriol. 179: 2717-2723, 1997. PubMed: 909807192096: Applied Biosystems MicroSeq Pharmaceutical Panel #1. :Applied Biosystems;MicroSeq92287: Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10.92409: Applied Biosystems MicroSeq PHL Panel #1. :Applied Biosystems;MicroSeq
常見問題
答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲，擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。
答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。
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