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產(chǎn)品詳情
Organism: Deinococcus radiodurans (ex Raj et al.) Brooks and Murray deposited asMicrococcus radiodurans Raj et al. Designations: R1 (smooth) [CCUG 27074, CIP 104750, DSM 20539, HAMBI 1924, IFO 15346, LMG 4051, UWO 288, VKM B-1422] Isolation: irradiated ground pork and beef Depositor: AW Anderson Biosafety Level: 1 耐放射異常球菌 ATCC 13939 Shipped: freeze-dried Growth Conditions: ATCC medium86: Nutrient agar with 1% glucose
Temperature: 30.0°C Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Cross References: Nucleotide (GenBank) : X52300 Deinococcus radiodurans 5S ribosomal RNA. Type Strain: yes [8522] Comments: cell wall morphology [6822]
radiation-resistant; withstands 6 x 10(6) rep.
Applications: produces restriction endonuclease MraI [8177] [58866] Related Products: purified DNA: ATCC 13939D-5 References: 6165: Food Technol. 10: 575, 1956.
6822: Karrenberg FH, et al. Surface structure variants in Deinococcus radiodurans. Curr. Microbiol. 16: 15-20, 1987.
8177: Wani AA, et al. A sequence specific endonuclease from Micrococcus radiodurans. Biochim. Biophys. Acta 697: 178-184, 1982. PubMed: 6285977
8522: Brooks BW, Murray RG. Nomenclature for "Micrococcus radiodurans" and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int. J. Syst. Bacteriol. 31: 353-360, 1981.
32542: Mattimore V, Battista JR. Radioresistance of Deinococcus radiodurans: functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J. Bacteriol. 178: 633-637, 1996. PubMed: 8550493
49793: White O, et al. Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science 286: 1571-1577, 1999. PubMed: 10567266
58866: Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885
常見問題
答:公司提供兩種運(yùn)輸方式供老師選擇,1、復(fù)蘇的活細(xì)胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟,細(xì)胞成活率較高。2、凍存的細(xì)胞:采用干冰運(yùn)輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲,擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點(diǎn)是需要自己復(fù)蘇。
答:我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進(jìn)行選擇!
答:不可以重復(fù)使用,一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多,通常在3-5%,以維持細(xì)胞存活,控制生長速度,不可以用來做細(xì)胞培養(yǎng)使用。
答:細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn),一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物,懸浮細(xì)胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常,白色的圓點(diǎn)是分散分布的,聚團(tuán)類的懸浮細(xì)胞可能會聚團(tuán)出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答:一般情況下,我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細(xì)胞數(shù)量多一些,便于后期復(fù)蘇。購買原代細(xì)胞的客戶,要充分考慮該細(xì)胞的傳代次數(shù)限制,人源原代細(xì)胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細(xì)胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。



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