產(chǎn)品推薦
聯(lián)系方式
- 電話:400-1016-218
- 郵箱:355185756@qq.com
- 地址:安徽省合肥市高新區(qū)黃山路602號合肥國家大學(xué)科技園A401室
產(chǎn)品詳情
Organism: Acinetobacter calcoaceticus (Beijerinck) Baumann et al. deposited as Moraxella calcoacetica Beijerinck Designations: 46 [1, IFO 13718] Depositor: M Doudoroff History: ATCC <<--M Doudoroff<<--Lab. Microbiol., Delft strain 1 (Micrococcus calcoaceticus) <<--- M.W. Beijerinck Biosafety Level: 2 Shipped: freeze-dried Growth Conditions: ATCC medium3: Nutrient agar or nutrient broth
Temperature: 30.0°C Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Cross References: Nucleotide (GenBank) : AB008685 Acinetobacter calcoaceticus DNA for DNA gyrase subunit B, partial cds, strain CIP 81.08.
Nucleotide (GenBank) : U60278 Acinetobacter calcoaceticus 16S-23S ribosomal RNA intergenic spacer, including Ile-tRNA and Ala-tRNA genes.
Nucleotide (GenBank) : D73427 Acinetobacter calcoaceticus gene for DNA gyrase subunit B protein, N terminal side, partial cds.
Nucleotide (GenBank) : Z93434 A.calcoaceticus 16S rRNA gene (strain ATCC 23055).
Nucleotide (GenBank) : D50330 Acinetobacter calcoaceticus genes for p III and acdp, partial and complete cds.
Type Strain: yes(type strain) Comments: Carried by Delft as strain 317 until 1922
taxonomy [8724]
乙酸鈣不動(dòng)桿菌 ATCC 23055 Applications: produces isoprene [7343] References: 7343: Kuzma J, et al. Bacteria produce the volatile hydrocarbon isoprene. Curr. Microbiol. 30: 97-103, 1995. PubMed: 7765889
8724: Bouvet PJ, Grimont PA. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int. J. Syst. Bacteriol. 36: 228-240, 1986.
10303: Baumann P, et al. A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J. Bacteriol. 95: 1520-1541, 1968. PubMed: 5650064
36887: Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980.
常見問題
答:公司提供兩種運(yùn)輸方式供老師選擇,1、復(fù)蘇的活細(xì)胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟,細(xì)胞成活率較高。2、凍存的細(xì)胞:采用干冰運(yùn)輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲,擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點(diǎn)是需要自己復(fù)蘇。
答:我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進(jìn)行選擇!
答:不可以重復(fù)使用,一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多,通常在3-5%,以維持細(xì)胞存活,控制生長速度,不可以用來做細(xì)胞培養(yǎng)使用。
答:細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn),一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物,懸浮細(xì)胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常,白色的圓點(diǎn)是分散分布的,聚團(tuán)類的懸浮細(xì)胞可能會聚團(tuán)出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答:一般情況下,我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細(xì)胞數(shù)量多一些,便于后期復(fù)蘇。購買原代細(xì)胞的客戶,要充分考慮該細(xì)胞的傳代次數(shù)限制,人源原代細(xì)胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細(xì)胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。



一鍵復(fù)制產(chǎn)品信息











皖公網(wǎng)安備 34010402703761號
夏經(jīng)理:341412994






