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產(chǎn)品詳情
NBRC 31327=ATCC 24449 里氏木霉
Organism:Trichoderma reesei Simmons, anamorph deposited as Trichoderma viride Persoon : Fries, anamorphDesignations:QM 9123 [CBS 439.92, IMI 192655, T.V. B115]Isolation:radiation mutant 207 of QM 6aDepositors:QM NBRC 31327=ATCC 24449 里氏木霉History:ATCC <<--QM<<--M. MandelsBiosafety Level:1Shipped:freeze-driedGrowth Conditions:ATCC medium 336: Potato dextrose agar （PDA）Temperature: 25.0°C Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Applications:produces cellulose 1,4-beta-cellobiosidase D cellobiohydrolase D [58920] produces endoglucanase [58929] produces fat [58252] produces glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase produces palmitic acid [15212] produces palmitoleic acid [15212] produces single-cell protein SCP [138] produces stearic acid [15212] produces intracellular beta-glucosidase [59153]Comments:enhanced cellulase production [17263] [47599] [58930] [58931]Subcollection:FungiReferences:138: Peitersen N. Cellulase and protein production from mixed cultures of Trichoderma viride and a yeast. Biotechnol. Bioeng. 17: 1291-1299, 1975. PubMed: 110197915212: . . Appl. Microbiol. Biotechnol. 34: 335-339, 1990.17263: . . J. Polym. Sci. Part C 36: 445-459, 1971.47599: Mandels M, Weber J. The production of cellulases. Adv. Chem. 95: 391-414, 1969.58252: et al., Brown DE. Fat production by Trichoderma reesei. Biotechnol. Lett. 10: 249-254, 1988.58920: Gum EK J, Brown RD Jr.. Comparison of four purified extracellular 1,4-beta-D-glucan cellobiohydrolase enzymes from Trichoderma viride. Biochim. Biophys. Acta 492: 225-231, 1977. PubMed: 40504858929: Peitersen N. Production of cellulase and protein from barley straw by Trichoderma viride. Biotechnol. Bioeng. 17: 361-374, 1975.58930: Mandels M, et al. Enhanced cellulase production by a mutant of Trichoderma viride. Appl. Microbiol. 21: 152-154, 1971. PubMed: 509976158931: Poincelot RP, Day PR. Simple dye release assay for determining cellulolytic activity of fungi. Appl. Microbiol. 23: 875-879, 1972. PubMed: 506409459153: Inglin M, et al. Partial purification and characterization of a new intracellular beta-glucosidase of Trichoderma reesei. Biochem. J. 185: 515-519, 1980. PubMed: 6772150 NBRC 31327=ATCC 24449
常見(jiàn)問(wèn)題
答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過(guò)夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。
答：我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶(hù)提供了替代培養(yǎng)方案，根據(jù)客戶(hù)的意愿進(jìn)行選擇！
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來(lái)做細(xì)胞培養(yǎng)使用。
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類(lèi)的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶(hù)收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買(mǎi)原代細(xì)胞的客戶(hù)，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。
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