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ATCC 700603 肺炎克雷伯菌

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貨號(hào)Delf-18167

規(guī)格 凍干粉/培養(yǎng)物

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400-1016-218
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安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室

產(chǎn)品詳情

Organism:	Klebsiella pneumoniae subsp. pneumoniae （Schroeter） Trevisan deposited as Klebsiella pneumoniae （Schroeter） Trevisan
Designations:	K6 [CCUG 45421, LMG 20218, MCV37]
Isolation:	urine from hospitalized patient, Richmond, VA, 1994 [110814]
Depositor:	JM Swenson
History:	ATCC <<--JM Swenson<<--M.J. Ferraro <<-- G. Jacoby <<-- H. Dalton
Biosafety Level:	2 ATCC 700603 肺炎克雷伯菌
Shipped:	freeze-dried
Growth Conditions:	ATCC medium3: Nutrient agar or nutrient broth Temperature: 37.0°C Duration: aerobic ATCC 700603
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cross References:	Nucleotide （GenBank） : AF132290 Klebsiella pneumoniae plasmid-encoded extended-spectrum beta-lactamase （bla SHV-18） gene, complete cds. Nucleotide （GenBank） : AX111170 Sequence 1903 from Patent WO0123604.
Comments:	MIC （ug/ml）: Ampicillin >64; Amoxicillin-clavulanate 8; Aztreonam 64; Aztreonam-clavulanate <=0.25; Cefotaxime 8; Cefotaxime-clavulanate <=0.25; Cefpodoxime 16; Cefpodoxime-clavulanate 1; Ceftazidime 32 [49425] MIC （ug/ml）: Ceftazidime-clavulanate 1; Ceftriaxone 16; Ceftriaxone-clavulanate <=0.25; cefepime="" cefoxitin="" chloramphenicol="">32; Ciprofloxacin 0.5; Gentamicin 8 [49425] MIC （ug/ml）: Imipenem <=1; piperacillin="">128; Piperacillin-tazobactam 16; Tetracycline 16; Tobramycin 4; Trimethoprim-sulfamethoxazole 2 [49425]
Applications:	produces beta-lactamase SHV-18 [49425] quality control strain [92137] [92160] resistant to ampicillin [49425] resistant to aztreonam [49425] resistant to cefoxitin [49425] resistant to cefpodoxime [49425] resistant to ceftazidime [49425] resistant to chloramphenicol [49425] resistant to piperacillin [49425] resistant to tetracycline [49425] sensitive to amoxicillin-clavulanate [49425] sensitive to cefepime [49425] sensitive to cef otaxime [49425] sensitive to ciprofloxacin [49425] sensitive to imipenem [49425] sensitive to piperacillin-tazobactam [49425] sensitive to tobramycin [49425] sensitive to trimethoprim-sulfamethoxazole [49425] susceptibility disc testing [21523] susceptibility testing [21525] [21533] [90283] [92219] intermediate resistance to ceftriaxone and gentamicin [49425] control for extended-spectrum beta-lactamase production [49425]
Related Products:	purified DNA: ATCC 700603D-5
References:	21523: CLSI Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA:Clinical and Laboratory Standards Institute;CLSI M2-A9. 21525: CLSI Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA:Clinical and Laboratory Standards Institute;CLSI M7-A7. 21533: CLSI Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA:Clinical and Laboratory Standards Institute;CLSI M31-A2. 49425: Rasheed JK, et al. Characterization of the extended-spectrum beta-lactamase reference strain, Klebsiella pneumoniae K6 （ATCC 700603）, which produces the novel enzyme SHV-18. Antimicrob. Agents Chemother. 44: 2382-2388, 2000. PubMed: 10952583 90283: CLSI Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA:Clinical and Laboratory Standards Institute;CLSI M100-S17, 2007 92137: BD Phoenix Gram Negative NMIC and NMIC/ID. :BD Becton Dickinson;Phoenix 92160: Sensititre MIC Susceptibility. :Trek Diagnostic Systems;Sensititre 92219: CLSI Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Informational Supplement. Wayne, PA:Clinical and Laboratory Standards Institute;CLSI M31-S1. 110814: Jana M Swenson, personal communication

常見問題

問：細(xì)胞的運(yùn)輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過(guò)夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。

問：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！

問：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來(lái)做細(xì)胞培養(yǎng)使用。

問：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問：剛買回來(lái)的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。