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ATCC 11778 蠟樣芽孢桿菌

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貨號(hào)Delf-18189

規(guī)格 凍干粉/培養(yǎng)物

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產(chǎn)品詳情

Preceptrol? CultureOrganism:Bacillus cereus Frankland and Frankland deposited as Bacillus mycoides FluggeDesignations:FDA strain PCI 213 [ATCC 9634, NCTC 10320, S.A. Waksman strain O]Depositor:FDABiosafety Level:1 ATCC 11778 蠟樣芽孢桿菌Shipped:freeze-driedGrowth Conditions:ATCC medium3: Nutrient agar or nutrient brothTemperature: 30.0°CPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Cross References:Nucleotide （GenBank） : AF238886 Bacillus cereus strain ATCC 11778 putative VrrB （vrrB） gene, partial cds.Nucleotide （GenBank） : U02893 Bacillus cereus ATCC 11778 16S rRNA gene, partial sequence.Applications:assay of chlortetracycline aureomycin [21742] assay of demeclocycline demethylchlortetracycline [11016] assay of doxycycline hydramycin [11016] [58602] assay of minocycline [58602] assay of oxytetracycline terramycin [21742] [58602] assay of tetracycline [58602] detection of [92834] media testing [92390] [92845] quality control strain testing [92374] quality control strain for BBL productsReferences:5618: Appl. Microbiol. 17: 91, 1969.5622: Proc. Soc. Exp. Biol. Med. 55: 66-69, 1944.11016: British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 200321742: AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23.32546: Demezas DH, Bell J. Evaluation of low molecular weight RNA profiles and ribotyping to differentiate some Bacillus species. Syst. Appl. Microbiol. 18: 582-589, 1995.58602: Simon HJ, Yin EJ. Microbioassay of antimicrobial agents. Appl. Microbiol. 19: 573-579, 1970. PubMed: 498672592374: Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin （ADA-V）. Washington, DC:Environmental Protection Agency;EPA EPA Method 1605, 200192390: Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva （Switzerland）:International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.92834: Microbiology of food and animal feeding stuffs --- Horizontal method for the determination of low numbers of presumptive Bacillus cereus --- Most probable number technique and detection method. London, UK:British Standards Institution;British Standard BS EN ISO 21871:2006.92845: Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

常見問題

問：細(xì)胞的運(yùn)輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。

問：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！

問：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。

問：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問：剛買回來的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。