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產(chǎn)品詳情
Mycobacterium smegmatis (Trevisan) Lehmann and Neumann (ATCC?607?)
|
Application |
Assay of bleomycin Bacteriophage host Pharmaceutical and Personal Care |
|
Isolation |
Human |
|
Type Strain |
no |
|
Biosafety Level |
1 |
|
Product Format |
freeze-dried |
|
Storage Conditions |
Frozen: -80°C or colder |
|
Preceptrol? |
no ATCC 607菌種, 恥垢分枝桿菌 |
|
Type Strain |
no |
|
Comments |
Avirulent Bacteriophage host Membrane lipids Parent of ATCC 700738 |
|
Medium |
ATCC? Medium 44: Brain Heart Infusion Agar/Broth |
|
Growth Conditions |
Temperature: 37°C Atmosphere: Aerobic |
|
Name of Depositor |
RD Herrold |
|
Chain of Custody |
ATCC <-- RD Herrold <-- F Novy (original Koch strain of Bacillus tuberculosis) |
|
Isolation |
Human |
|
References |
Gordon RE, Smith MM. Rapidly growing, acid fast bacteria. I. Species' descriptions of Mycobacterium phlei Lehmann and Neumann and Mycobacterium smegmatis (Trevisan) Lehmann and Neumann. J. Bacteriol. 66: 41-48, 1953. PubMed: 13069464 Science 75: 513, 1932. Science 105: 435, 1947. . . Am. Rev. Tuberc. 58: 697, 1948. . . Curr. Microbiol. 7: 49-51, 1982. British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003 European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 U.S. Pharmacopeia General Chapters;<81>Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 Microbial assay for antibiotics. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.34. Charlotte M McCarthy, personal communication |
|
Cross References |
Nucleotide (GenBank) : X60513 M.smegmatis gltA gene for citrate synthase. Nucleotide (GenBank) : U07954 Mycobacterium smegmatis ATCC 607 16S rRNA gene, 5' untranslated region. Nucleotide (GenBank) : AH000830 Mycobacterium smegmatis ATCC 607 16S rRNA gene, 5' untranslated region. Nucleotide (GenBank) : U07955 Mycobacterium smegmatis ATCC 607 intergenic region between 16S and 23S rRNA genes. Nucleotide (GenBank) : U87307 Mycobacterium smegmatis extracytoplasmic function alternative sigma factor (sigE) gene, complete cds. |
常見問題
答:公司提供兩種運(yùn)輸方式供老師選擇,1、復(fù)蘇的活細(xì)胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟,細(xì)胞成活率較高。2、凍存的細(xì)胞:采用干冰運(yùn)輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲,擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點(diǎn)是需要自己復(fù)蘇。
答:我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進(jìn)行選擇!
答:不可以重復(fù)使用,一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多,通常在3-5%,以維持細(xì)胞存活,控制生長速度,不可以用來做細(xì)胞培養(yǎng)使用。
答:細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn),一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物,懸浮細(xì)胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常,白色的圓點(diǎn)是分散分布的,聚團(tuán)類的懸浮細(xì)胞可能會聚團(tuán)出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答:一般情況下,我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細(xì)胞數(shù)量多一些,便于后期復(fù)蘇。購買原代細(xì)胞的客戶,要充分考慮該細(xì)胞的傳代次數(shù)限制,人源原代細(xì)胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細(xì)胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。



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