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ATCC 19606菌種，鮑曼不動(dòng)桿菌

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貨號(hào)Delf-18259

規(guī)格 凍干粉/培養(yǎng)物

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產(chǎn)品詳情

Acinetobacter baumannii Bouvet and Grimont (ATCC? 19606?)

Deposited As	Bacterium anitratum Schaub and Hauber
Strain Designations	2208 [81, DSM 6974]
Application	Media testing Quality control strain Quality control strain for bioMerieux Vitek and Sensititre products Food testing
Isolation	Urine
Type Strain	yes
Biosafety Level	2 ATCC 19606菌種，鮑曼不動(dòng)桿菌
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol?	yes
Type Strain	yes
Medium	ATCC? Medium 3: Nutrient agar or nutrient broth
Growth Conditions	Temperature: 37°C Atmosphere: Aerobic
Name of Depositor	R Hugh
Chain of Custody	ATCC <-- R Hugh <-- EO King <-- IG Schaub 81
Isolation	Urine
References	Hugh R, Reese R. Designation of the type strain for Bacterium anitratum Schaub and Hauber 1948. Int. J. Syst. Bacteriol. 17: 245-254, 1967. Schaub IG, Hauber FD. A biochemical and serological study of a group of identical unidentifiable gram negative bacilli from human sources. J. Bacteriol. 56: 379-385, 1948. Hugh R, Reese R. A comparison of 120 strains of Bacterium anitratum Schaub and Hauber with the type strain of this species. Int. J. Syst. Bacteriol. 18: 207-229, 1968. Bouvet PJ, Grimont PA. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int. J. Syst. Bacteriol. 36: 228-240, 1986. Listeria species -- Biochemical Identification Method (Vitek GPI and GNI+). Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 992.19. Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10. RapID? NF PLUS QC Set. Remel?.
Cross References	Nucleotide (GenBank) : AX111482 Sequence 2215 from Patent WO0123604. Nucleotide (GenBank) : AX111503 Sequence 2236 from Patent WO0123604. Nucleotide (GenBank) : AX111523 Sequence 2256 from Patent WO0123604. Nucleotide (GenBank) : Z93435 Acinetobacter sp. 16S rRNA gene (strain ATCC 19606). Nucleotide (GenBank) : Z96926 Acinetobacter baumannii waaA gene, strain ATCC 19606. Nucleotide (GenBank) : AF100557 Acinetobacter baumannii DNA gyrase A (gyrA) gene, partial cds. Nucleotide (GenBank) : AB008684 Acinetobacter baumannii DNA for DNA gyrase subunit B, partial cds, strain CIP 70.34. Nucleotide (GenBank) : D73413 Acinetobacter baumannii gene for DNA gyrase subunit B protein, C terminal side, partial cds. Nucleotide (GenBank) : D73428 Acinetobacter baumannii gene for DNA gyrase subunit B protein, N terminal side, partial cds. Nucleotide (GenBank) : U60279 Acinetobacter baumannii 16S-23S ribosomal RNA intergenic spacer, including Ile-tRNA and Ala-tRNA genes. Nucleotide (GenBank) : AB001599 Acinetobacter baumannii DNA for L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase, complete cds.

常見問(wèn)題

問(wèn)：細(xì)胞的運(yùn)輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過(guò)夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。

問(wèn)：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！

問(wèn)：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來(lái)做細(xì)胞培養(yǎng)使用。

問(wèn)：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問(wèn)：剛買回來(lái)的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。