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產(chǎn)品詳情
Streptococcus mutans Clarke (ATCC? 700610?)
|
Strain Designations |
UA159 [UAB577] |
|
Application |
Transformation host |
|
Isolation |
Child with active caries, 1982 |
|
Type Strain |
no |
|
Biosafety Level |
1 |
|
Antigenic Properties |
Serotype c |
|
Product Format |
freeze-dried |
|
Storage Conditions |
Frozen: -80°C or colder |
|
Preceptrol? |
no |
|
Genome Sequenced Strain |
Streptococcus mutans UA159, complete genome. |
|
Type Strain |
no |
|
Antigenic Properties |
Serotype c |
|
Antibiotic Resistance |
Sensitive to erythromycin Sensitive to rifampin rifampicin, rifamycin AMP Sensitive to spectinomycin actinospectacin Sensitive to streptomycin |
|
Comments |
Genome sequenced strain sensitive to erythromycin sensitive to rifampin rifampicin, rifamycin AMP sensitive to spectinomycin actinospectacin sensitive to streptomycin |
|
Medium |
ATCC? Medium 44: Brain Heart Infusion Agar/Broth ATCC ? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
|
Growth Conditions |
Temperature: 37°C Atmosphere: 5% CO2 or anaerobic |
|
Name of Depositor |
PW Caufield |
|
Isolation |
Child with active caries, 1982 |
|
References |
Burne RA, et al. Cariogenicity of Streptococcus mutans strains with defects in fructan metabolism assessed in a program-fed specific-pathogen-free rat model. J. Dent. Res. 75: 1572-1577, 1996. PubMed: 8906125 Quivey RG Jr., et al. Acid adaptation in Streptococcus mutans UA159 alleviates sensitization to environmental stress due to RecA deficiency. FEMS Microbiol. Lett. 126: 257-261, 1995. PubMed: 7729669 Tao L, et al. Transformation efficiency of EMS-induced mutants of Streptococcus mutans of altered cell shape. J. Dent. Res. 72: 1032-1039, 1993. PubMed: 8496476 Wexler DL, et al. Characteristics and cariogenicity of a fructanase-defective Streptococcus mutants strain. Infect. Immun. 60: 3673-3681, 1992. PubMed: 1500176 Murchison HH, et al. Transformation of Streptococcus mutans with chromosomal and shuttle plasmid (pYA629) DNAs. Infect. Immun. 54: 273-282, 1986. PubMed: 3021626 Ajdic D, et al. Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen.. Proc. Natl. Acad. Sci. USA 99: 14434-14439, 2002. PubMed: 12397186 Page W Caufield, personal communication |
|
Cross References |
Nucleotide (GenBank) : AE014133 Streptococcus mutans UA159, complete genome. |
常見(jiàn)問(wèn)題
答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過(guò)夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。
答：我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來(lái)做細(xì)胞培養(yǎng)使用。
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。
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