A1143 NRRL 3
目錄價
￥ 0
一鍵復制產(chǎn)品信息
別稱Aspergillus Niger Van Tieghem
貨號Delf-17256
規(guī)格
產(chǎn)品推薦
聯(lián)系方式
- 電話：400-1016-218
- 郵箱：355185756@qq.com
- 地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學科技園A401室
產(chǎn)品詳情
Aspergillus niger van Tieghem 拉丁名
(ATCC? 9029?) 統(tǒng)一編號
Strain Designations 別名 A1143 NRRL 3 [3; 320, CBS 120.49, DSM 2466, IMI 41876, N400, NRRL 566, WB 3, WB 566]
Application 用途 Produces 4-nitrophenylphosphatase；
Produces D-xylulose dehydrogenase；
Produces L-malic acid；
Produces L-xylulose reductase；
Produces acid protease protease (acid)；
Produces alpha-L-arabinofuranosidase A；
Produces alpha-L-arabinofuranosidase B；
Produces citric acid citrate；
Produces endo-arabinase；
Produces ferulic acid esterase；
Produces gluconic acid；
Produces glucono-delta-lactone；
Produces glucose oxidase；
Produces glucuronidase, beta beta-glucuronidase；
Produces lincomycin sulfoxides；
Produces urease urea amidolyase；
Transformation host；
Degrades: pulp and paper-mill wastewater；
Produces citric acid from whey permeate；
Produces gluconic acid in the production of aldonic acid and； aldonate compositions useful in cleaning solutions；
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏條件
Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌種 no
Preceptrol? no
Genome Sequenced Strain Yes
Comments 注釋 Enhancement of glucose oxidase production by addition of hydrocarbons
Genome sequencing strain (Integrated Genomics Inc, USA).
Medium 培養(yǎng)基 ATCC? Medium 325: Malt extract agar (Blakeslee's formula)
ATCC? Medium 200: YM agar or YM broth
ATCC? Medium 336: Potato dextrose agar (PDA)
Growth Conditions 生長條件
Temperature: 24°C to 26°C
Atmosphere 需氧情況: Typical aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGC
GGGTCCTTTGGGCCCAACCTCCCATCCGTGTCTATTGTACCCT
GTTGCTTCGGCGGGCCCGCCGCTTGTCGGCCGCCGGGGGGG
CGCCTCTGCCCCCCGGGCCCGTGCCCGCCGGAGACCCCAAC
ACGAACACTGTCTGAAAGCGTGCAGTCTGAGTTGATTGAATGC
AATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATC
GATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCA
GAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCC
CTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGC
CCTCAAGCCCGGCTTGTGTGTTGGGTCGCCGTCCCCCTCTCCG
GGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGA
TCCTCGAGCGTATGGGGCTTTGTCACATGCTCTGTAGGATTGG
CCGGCGCCTGCCGACGTTTTCCAACCATTCTTTCCAGGTTGAC
CTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCA
ATAA
D1D2 region of the 28S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTC
AGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGC
TGGCTCCTTCGGAGTCCGCATTGTAATTTGCAGAGGATGCTTT
GGGTGCGGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCAG
AGAGGGTGAGAATCCCGTCTTGGGCGGGGTGTCCGTGCCCG
TGTAAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGC
TCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCG
GAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAA
GCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAA
AGGGAAGCGCTTGCGACCAGACTCGCCCGCGGGGTTCAGC
CGGCATTCGTGCCGGTGTACTTCCCCGTGGGCGGGCCAGCG
TCGGTTTGGGCGGCCGGTCAAAGGCCCCTGGAATGTAGTGC
CCTCCGGGGCACCTTATAGCCAGGGGTGCAATGCGGCCAGC
CTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAAT
GGTCGTAAACGAC
beta-tubulin gene
TTGCCCTCCCCGTCCCTCGTCCGTCAGGAGACGCGTCGTTG
GTTGGCATCTCTTTTGCTCGGGACCCCACCGGTTCTTCGACCA
ACTCATTCTTGTGCTAACTGCATGTCTTCTTCGCTTCATAGGTTC
ACCTCCAAACCGGCCAGTGTGTAAGTGCCAATATATGCTTCG
GATGATTGCCCCCAAGGGTCTTGATTGGTGTTTGGTGGACTAA
ACAATATATCATGGTGGTTAGGGTAACCAAATTGGTGCTGCTT
TCTGGTACGTATACAACTGCCATTGGATTGGGGATGGAACAT
CGTCTCTTAGGCTATCTCAGCTTGAGTTCAGATGTTGTCCATTA
GGTACATGCTATCGGTCTAAGAACACGTCTAACAATTCAACA
GGCAGACCATCTCTGGCGAGCACGGCCTTGACGGCTCCGGT
GTGTAAGTGCAACTTTTTCACACCTCTCAATTGGTCAACAATG
GGCAAAGGGTTGGGTCTTCTGACACGCAGGATAGTTACAATG
GCACCTCCGACCTCCAGCTGGAGCGCATGAACGTCTACTTCA
ACGAGGTGAGATCCATCGGACCTTGGCTTTTTCACGACAATAT
CATCAATGTCCTAATCACTTCAGCAGGCTAGCGGTAACAAGT
ATGTTCCTCGTGCCGTCCTCGTCGACCTCGAGCCCGGTACCA
TGGACGCCGTCCGTGCCGGTCCTTTCGGCCAGCTCTTCCGCC
CCGACAACTTCGTCTTCGGCCAGTCCGGTGCTGGTAACAACT
GG
Name of Depositor 寄存者 NRRL
Chain of Custody 來源國家 ATCC <-- NRRL <-- Moyer 3 <-- Anthony 320
Cross References Nucleotide (GenBank) : KU729033 ITS including 5.8S rRNA gene
Nucleotide (GenBank) : KU729117 D1/D2 region of 28S rRNA gene
Nucleotide (GenBank) : KU897006 beta-tubulin gene
Nucleotide (GenBank) : X58892 polygalacturonase gene, pgaI
Nucleotide (GenBank) : X55784 pectin lyase gene, pelA, 5' end
Nucleotide (GenBank) : J05242 A.niger glucose oxidase mRNA, complete cds.
Nucleotide (GenBank) : Z26938 A.niger cprA gene for NADPH cytochrome P450 oxidoreductase.
Nucleotide (GenBank) : L19059 subtilisin-like protease gene, pepD, complete coding sequence
References 參考文獻 Bercovitz A, et al. Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains. Appl. Environ. Microbiol. 56: 1594-1597, 1990. PubMed: 2383004
Versaw WK, et al. Purification and properties of a 4-nitrophenylphosphatase from Aspergillus niger. Arch. Biochem. Biophys. 287: 85-90, 1991. PubMed: 1654806
Faulds CB, Williamson G. Purification and characterization of a ferulic acid esterase (FAE-III) from Aspergillus niger: specificity for the phenolic moiety and binding to microcrystalline cellulose. Microbiology 140: 779-787, 1994.
Witteveen CF, et al. Isolation and characterization of two xylitol dehydrogenases from Aspergillus niger. Microbiology 140: 1679-1685, 1994.
Ziffer J, et al. Aldonic acid and aldonate compositions and production thereof. US Patent 3,454,501 dated Jul 8 1969
Argoudelis AD, Mason DJ. Production of lincomycin sulfoxide. US Patent 3,616,244 dated Oct 26 1971
Lakshminarayanan K. Method for the production of glucose oxidase. US Patent 3,701,715 dated Oct 31 1972
Storm AM, Gasner LL. Treatment of pulp mill wastes. US Patent 3,737,374 dated Jun 5 1973
Peppler HJ. Microbial technology. New York: Reinhold; 1967.
v.d. Veen P, et al. Induction, purification and characterisation of arabinases produced by Aspergillus niger. Arch. Microbiol. 157: 23-28, 1991. PubMed: 1814275
Jarai G, et al. Cloning and characterization of the pepD gene of Aspergillus niger which codes for a subtilisin-like protease. Gene 139: 51-57, 1994. PubMed: 8112588
Harmsen JA, et al. Cloning and expression of a second Aspergillus niger pectin lyase gene (pelA): indications of a pectin lyase gene family in A. niger. Curr. Genet. 18: 161-166, 1990. PubMed: 2225145
Bussink HJ, et al. Identification and characterization of a second polygalacturonase gene of Aspergillus niger. Curr. Genet. 20: 301-307, 1991. PubMed: 1934135
Jarai G, Buxton F. Nitrogen, carbon, and pH regulation of extracellular acidic proteases of Aspergillus niger. Curr. Genet. 26: 238-244, 1994. PubMed: 7532112
Li TH, Chen TL. Enhancement of glucose oxidase fermentation by addition of hydrocarbons. J. Ferment. Bioeng. 78: 298-303, 1994.
Gottschalk TE, et al. Detection of endogenous beta-glucuronidase activity in Aspergillus niger. Appl. Microbiol. Biotechnol. 45: 240-244, 1996. PubMed: 8920195
Smith PT, et al. Isolation and characterization of urease from Aspergillus niger. J. Gen. Microbiol. 139: 957-962, 1993. PubMed: 8336111
Faulds CB, Williamson G. Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE- III) from Aspergillus niger. Appl. Microbiol. Biotechnol. 43: 1082-1087, 1995. PubMed: 8590660
Frederick KR, et al. Glucose oxidase from Aspergillus niger. Cloning, gene sequence, secretion from Saccharomyces cerevisiae and kinetic analysis of a yeast-derived enzyme [published erratum appears in J. Biol. Chem. 265: 11405, 1990]. J. Biol. Chem. 265: 3793-3802, 1990. PubMed: 2406261
Khanh NQ, et al. Effects of promoters on the enhancement of pectin methyl esterase expression in Aspergillus niger. Biotechnol. Lett. 14: 1047-1052, 1992.
Dronawat SN, et al. The effects of agitation and aeration on the production of gluconic acid by Aspergillus niger. Appl. Biochem. Biotechnol. 51/52: 347-354, 1995.
. . Egypt J. Food Sci. 5: 9-20, 1977.
Murray FR, et al. Isolation of the glucose oxidase gene from Talaromyces flavus and characterisation of its role in the biocontrol of Verticillium dahliae. Curr. Genet. 32: 367-375, 1997. PubMed: 9371889
. . Egypt J. Food Sci. 5: 21-29, 1977.
Blom RH, et al. Sodium gluconate production. Fermentation with Aspergillus niger. Ind. Eng. Chem. 44: 435-440, 1952.
Somkuti GA, Bencivengo MM. Citric acid fermentation in whey permeate. Dev. Ind. Microbiol. 22: 557-563, 1981.
Andersen MR, et al. Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88. Genome Res. 21: 885-897, 2011. PubMed: 21543515
Howard SJ, et al. Cryptic species and azole resistance in the Aspergillus niger complex. Antimicrob Agents Chemother 55: 4802-4809, 2011. PubMed: 21768508
Martens-Uzunova ES, Schaap PJ. An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation. Fungal Genet Biol 45: 1449-1457, 2008. PubMed: 18768163
Peterson SW. Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. Mycologia 100: 205-226, 2008. PubMed: 18595197
Geiser DM, et al. The current status of species recognition and identification in Aspergillus. Stud Mycol 59: 1-10, 2007. PubMed: 18490947
Semova N, et al. Generation, annotation, and analysis of an extensive Aspergillus niger EST collection. BMC Microbiol 6: 7-7, 2006. PubMed: 16457709
Basten DE, et al. Characterisation of Aspergillus niger prolyl aminopeptidase. Mol Genet Genomics 272: 673-679, 2005. PubMed: 15654623
Poulsen BR, et al. Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger. FEBS J 272: 1313-1325, 2005. PubMed: 15752350
Basten DEJW, Dekker PJT, Schaap PJ. Aminopeptidase C of Aspergillus niger is a novel phenylalanine aminopeptidase. Appl Environ Microbiol 69: 1246-1250, 2003. PubMed: 12571053
de Vries RP, et al. Regulation of the alpha-glucuronidase-encoding gene (aguA) from Aspergillus niger. Mol Genet Genomics 268: 96-102, 2002. PubMed: 12242504
de Vries RP, et al. The Aspergillus niger faeB gene encodes a second feruloyl esterase involved in pectin and xylan degradation, and is specifically induced on aromatic compounds. J Biochem 36: 377-386, 2002. PubMed: 11931668
de Vries RP, et al. The beta-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions. Eur J Biochem 269: 4985-4993, 2002. PubMed: 12383257
de Vries RP, et al. Expression profiling of pectinolytic genes from Aspergillus niger. FEBS Lett 530: 41-47, 2002. PubMed: 12387863
(ATCC? 9029?) 統(tǒng)一編號
Strain Designations 別名 A1143 NRRL 3 [3; 320, CBS 120.49, DSM 2466, IMI 41876, N400, NRRL 566, WB 3, WB 566]
Application 用途 Produces 4-nitrophenylphosphatase；
Produces D-xylulose dehydrogenase；
Produces L-malic acid；
Produces L-xylulose reductase；
Produces acid protease protease (acid)；
Produces alpha-L-arabinofuranosidase A；
Produces alpha-L-arabinofuranosidase B；
Produces citric acid citrate；
Produces endo-arabinase；
Produces ferulic acid esterase；
Produces gluconic acid；
Produces glucono-delta-lactone；
Produces glucose oxidase；
Produces glucuronidase, beta beta-glucuronidase；
Produces lincomycin sulfoxides；
Produces urease urea amidolyase；
Transformation host；
Degrades: pulp and paper-mill wastewater；
Produces citric acid from whey permeate；
Produces gluconic acid in the production of aldonic acid and； aldonate compositions useful in cleaning solutions；
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏條件
Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌種 no
Preceptrol? no
Genome Sequenced Strain Yes
Comments 注釋 Enhancement of glucose oxidase production by addition of hydrocarbons
Genome sequencing strain (Integrated Genomics Inc, USA).
Medium 培養(yǎng)基 ATCC? Medium 325: Malt extract agar (Blakeslee's formula)
ATCC? Medium 200: YM agar or YM broth
ATCC? Medium 336: Potato dextrose agar (PDA)
Growth Conditions 生長條件
Temperature: 24°C to 26°C
Atmosphere 需氧情況: Typical aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGC
GGGTCCTTTGGGCCCAACCTCCCATCCGTGTCTATTGTACCCT
GTTGCTTCGGCGGGCCCGCCGCTTGTCGGCCGCCGGGGGGG
CGCCTCTGCCCCCCGGGCCCGTGCCCGCCGGAGACCCCAAC
ACGAACACTGTCTGAAAGCGTGCAGTCTGAGTTGATTGAATGC
AATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATC
GATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCA
GAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCC
CTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGC
CCTCAAGCCCGGCTTGTGTGTTGGGTCGCCGTCCCCCTCTCCG
GGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGA
TCCTCGAGCGTATGGGGCTTTGTCACATGCTCTGTAGGATTGG
CCGGCGCCTGCCGACGTTTTCCAACCATTCTTTCCAGGTTGAC
CTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCA
ATAA
D1D2 region of the 28S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTC
AGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGC
TGGCTCCTTCGGAGTCCGCATTGTAATTTGCAGAGGATGCTTT
GGGTGCGGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCAG
AGAGGGTGAGAATCCCGTCTTGGGCGGGGTGTCCGTGCCCG
TGTAAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGC
TCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCG
GAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAA
GCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAA
AGGGAAGCGCTTGCGACCAGACTCGCCCGCGGGGTTCAGC
CGGCATTCGTGCCGGTGTACTTCCCCGTGGGCGGGCCAGCG
TCGGTTTGGGCGGCCGGTCAAAGGCCCCTGGAATGTAGTGC
CCTCCGGGGCACCTTATAGCCAGGGGTGCAATGCGGCCAGC
CTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAAT
GGTCGTAAACGAC
beta-tubulin gene
TTGCCCTCCCCGTCCCTCGTCCGTCAGGAGACGCGTCGTTG
GTTGGCATCTCTTTTGCTCGGGACCCCACCGGTTCTTCGACCA
ACTCATTCTTGTGCTAACTGCATGTCTTCTTCGCTTCATAGGTTC
ACCTCCAAACCGGCCAGTGTGTAAGTGCCAATATATGCTTCG
GATGATTGCCCCCAAGGGTCTTGATTGGTGTTTGGTGGACTAA
ACAATATATCATGGTGGTTAGGGTAACCAAATTGGTGCTGCTT
TCTGGTACGTATACAACTGCCATTGGATTGGGGATGGAACAT
CGTCTCTTAGGCTATCTCAGCTTGAGTTCAGATGTTGTCCATTA
GGTACATGCTATCGGTCTAAGAACACGTCTAACAATTCAACA
GGCAGACCATCTCTGGCGAGCACGGCCTTGACGGCTCCGGT
GTGTAAGTGCAACTTTTTCACACCTCTCAATTGGTCAACAATG
GGCAAAGGGTTGGGTCTTCTGACACGCAGGATAGTTACAATG
GCACCTCCGACCTCCAGCTGGAGCGCATGAACGTCTACTTCA
ACGAGGTGAGATCCATCGGACCTTGGCTTTTTCACGACAATAT
CATCAATGTCCTAATCACTTCAGCAGGCTAGCGGTAACAAGT
ATGTTCCTCGTGCCGTCCTCGTCGACCTCGAGCCCGGTACCA
TGGACGCCGTCCGTGCCGGTCCTTTCGGCCAGCTCTTCCGCC
CCGACAACTTCGTCTTCGGCCAGTCCGGTGCTGGTAACAACT
GG
Name of Depositor 寄存者 NRRL
Chain of Custody 來源國家 ATCC <-- NRRL <-- Moyer 3 <-- Anthony 320
Cross References Nucleotide (GenBank) : KU729033 ITS including 5.8S rRNA gene
Nucleotide (GenBank) : KU729117 D1/D2 region of 28S rRNA gene
Nucleotide (GenBank) : KU897006 beta-tubulin gene
Nucleotide (GenBank) : X58892 polygalacturonase gene, pgaI
Nucleotide (GenBank) : X55784 pectin lyase gene, pelA, 5' end
Nucleotide (GenBank) : J05242 A.niger glucose oxidase mRNA, complete cds.
Nucleotide (GenBank) : Z26938 A.niger cprA gene for NADPH cytochrome P450 oxidoreductase.
Nucleotide (GenBank) : L19059 subtilisin-like protease gene, pepD, complete coding sequence
References 參考文獻 Bercovitz A, et al. Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains. Appl. Environ. Microbiol. 56: 1594-1597, 1990. PubMed: 2383004
Versaw WK, et al. Purification and properties of a 4-nitrophenylphosphatase from Aspergillus niger. Arch. Biochem. Biophys. 287: 85-90, 1991. PubMed: 1654806
Faulds CB, Williamson G. Purification and characterization of a ferulic acid esterase (FAE-III) from Aspergillus niger: specificity for the phenolic moiety and binding to microcrystalline cellulose. Microbiology 140: 779-787, 1994.
Witteveen CF, et al. Isolation and characterization of two xylitol dehydrogenases from Aspergillus niger. Microbiology 140: 1679-1685, 1994.
Ziffer J, et al. Aldonic acid and aldonate compositions and production thereof. US Patent 3,454,501 dated Jul 8 1969
Argoudelis AD, Mason DJ. Production of lincomycin sulfoxide. US Patent 3,616,244 dated Oct 26 1971
Lakshminarayanan K. Method for the production of glucose oxidase. US Patent 3,701,715 dated Oct 31 1972
Storm AM, Gasner LL. Treatment of pulp mill wastes. US Patent 3,737,374 dated Jun 5 1973
Peppler HJ. Microbial technology. New York: Reinhold; 1967.
v.d. Veen P, et al. Induction, purification and characterisation of arabinases produced by Aspergillus niger. Arch. Microbiol. 157: 23-28, 1991. PubMed: 1814275
Jarai G, et al. Cloning and characterization of the pepD gene of Aspergillus niger which codes for a subtilisin-like protease. Gene 139: 51-57, 1994. PubMed: 8112588
Harmsen JA, et al. Cloning and expression of a second Aspergillus niger pectin lyase gene (pelA): indications of a pectin lyase gene family in A. niger. Curr. Genet. 18: 161-166, 1990. PubMed: 2225145
Bussink HJ, et al. Identification and characterization of a second polygalacturonase gene of Aspergillus niger. Curr. Genet. 20: 301-307, 1991. PubMed: 1934135
Jarai G, Buxton F. Nitrogen, carbon, and pH regulation of extracellular acidic proteases of Aspergillus niger. Curr. Genet. 26: 238-244, 1994. PubMed: 7532112
Li TH, Chen TL. Enhancement of glucose oxidase fermentation by addition of hydrocarbons. J. Ferment. Bioeng. 78: 298-303, 1994.
Gottschalk TE, et al. Detection of endogenous beta-glucuronidase activity in Aspergillus niger. Appl. Microbiol. Biotechnol. 45: 240-244, 1996. PubMed: 8920195
Smith PT, et al. Isolation and characterization of urease from Aspergillus niger. J. Gen. Microbiol. 139: 957-962, 1993. PubMed: 8336111
Faulds CB, Williamson G. Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE- III) from Aspergillus niger. Appl. Microbiol. Biotechnol. 43: 1082-1087, 1995. PubMed: 8590660
Frederick KR, et al. Glucose oxidase from Aspergillus niger. Cloning, gene sequence, secretion from Saccharomyces cerevisiae and kinetic analysis of a yeast-derived enzyme [published erratum appears in J. Biol. Chem. 265: 11405, 1990]. J. Biol. Chem. 265: 3793-3802, 1990. PubMed: 2406261
Khanh NQ, et al. Effects of promoters on the enhancement of pectin methyl esterase expression in Aspergillus niger. Biotechnol. Lett. 14: 1047-1052, 1992.
Dronawat SN, et al. The effects of agitation and aeration on the production of gluconic acid by Aspergillus niger. Appl. Biochem. Biotechnol. 51/52: 347-354, 1995.
. . Egypt J. Food Sci. 5: 9-20, 1977.
Murray FR, et al. Isolation of the glucose oxidase gene from Talaromyces flavus and characterisation of its role in the biocontrol of Verticillium dahliae. Curr. Genet. 32: 367-375, 1997. PubMed: 9371889
. . Egypt J. Food Sci. 5: 21-29, 1977.
Blom RH, et al. Sodium gluconate production. Fermentation with Aspergillus niger. Ind. Eng. Chem. 44: 435-440, 1952.
Somkuti GA, Bencivengo MM. Citric acid fermentation in whey permeate. Dev. Ind. Microbiol. 22: 557-563, 1981.
Andersen MR, et al. Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88. Genome Res. 21: 885-897, 2011. PubMed: 21543515
Howard SJ, et al. Cryptic species and azole resistance in the Aspergillus niger complex. Antimicrob Agents Chemother 55: 4802-4809, 2011. PubMed: 21768508
Martens-Uzunova ES, Schaap PJ. An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation. Fungal Genet Biol 45: 1449-1457, 2008. PubMed: 18768163
Peterson SW. Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. Mycologia 100: 205-226, 2008. PubMed: 18595197
Geiser DM, et al. The current status of species recognition and identification in Aspergillus. Stud Mycol 59: 1-10, 2007. PubMed: 18490947
Semova N, et al. Generation, annotation, and analysis of an extensive Aspergillus niger EST collection. BMC Microbiol 6: 7-7, 2006. PubMed: 16457709
Basten DE, et al. Characterisation of Aspergillus niger prolyl aminopeptidase. Mol Genet Genomics 272: 673-679, 2005. PubMed: 15654623
Poulsen BR, et al. Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger. FEBS J 272: 1313-1325, 2005. PubMed: 15752350
Basten DEJW, Dekker PJT, Schaap PJ. Aminopeptidase C of Aspergillus niger is a novel phenylalanine aminopeptidase. Appl Environ Microbiol 69: 1246-1250, 2003. PubMed: 12571053
de Vries RP, et al. Regulation of the alpha-glucuronidase-encoding gene (aguA) from Aspergillus niger. Mol Genet Genomics 268: 96-102, 2002. PubMed: 12242504
de Vries RP, et al. The Aspergillus niger faeB gene encodes a second feruloyl esterase involved in pectin and xylan degradation, and is specifically induced on aromatic compounds. J Biochem 36: 377-386, 2002. PubMed: 11931668
de Vries RP, et al. The beta-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions. Eur J Biochem 269: 4985-4993, 2002. PubMed: 12383257
de Vries RP, et al. Expression profiling of pectinolytic genes from Aspergillus niger. FEBS Lett 530: 41-47, 2002. PubMed: 12387863
常見問題
問：細胞的運輸方式有哪些？有什么區(qū)別？
答：公司提供兩種運輸方式供老師選擇，1、復蘇的活細胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復蘇的步驟，細胞成活率較高。2、凍存的細胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉入液氮長期存儲，擇機復蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復蘇。
問：為什么你們的細胞和其他公司的細胞培養(yǎng)條件不一樣？
答：我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進行選擇！
問：培養(yǎng)瓶的培養(yǎng)液可以重復使用嗎？
答：不可以重復使用，一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細胞存活，控制生長速度，不可以用來做細胞培養(yǎng)使用。
問：培養(yǎng)細胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點是什么？
答：細胞在鏡下發(fā)現(xiàn)圓形的白色的點點，一般情況下是為貼壁的細胞或脫落的細胞死亡后的產(chǎn)物，懸浮細胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團類的懸浮細胞可能會聚團出現(xiàn)白色的亮斑，技術老師可以繼續(xù)培養(yǎng)并觀察。
問：剛買回來的細胞如何凍存留種呢？
答：一般情況下，我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細胞數(shù)量多一些，便于后期復蘇。購買原代細胞的客戶，要充分考慮該細胞的傳代次數(shù)限制，人源原代細胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細胞，不建議凍存，收到后調整狀態(tài)后即可安排實驗。
皖公網(wǎng)安備 34010402703761號
方經(jīng)理：355185756
