一鍵復制產(chǎn)品信息
產(chǎn)品推薦
聯(lián)系方式
- 電話：400-1016-218
- 郵箱：355185756@qq.com
- 地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學科技園A401室
產(chǎn)品詳情
Organism: Plasmodium inui Halberstaedter and von Prowazek
Designations: Mulligan
Isolation: Macaca irus (M. fascicularis), Malaysia, pre-1934
Depositors: National Institutes of Health
History: ATCC<<--National Institutes of Health<<--Malaria Inst. India, New Delhi 1963 <<--- . . . <<--- H.W. Mulligan
Biosafety Level:2
Shipped: frozen
Growth Conditions: Duration: in vivo, Macaca mulatta Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: transmission by anopheline mosquitoes [38772] taxonomy, morphology, life cycle, and geographical distribution [4956] phylogeny [38801]
Classification: KINGDOM: Protozoa
References: 4859: Collins WE, et al. Plasmodium inui: serologic relationships of Asian isolates. Exp. Parasitol. 27: 507-515, 1970. PubMed: 4986810 4956: Eyles DE. The species of simian malaria: taxonomy, morphology, life cycle, and geographical distribution of the monkey species. J. Parasitol. 49: 866-887, 1963. PubMed: 14084190 5131: Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971. 38772: Collins WE, et al. Some observations on the transmission of Plasmodium inui. J. Parasitol. 54: 846-847, 1968. PubMed: 4990409 38801: Kissinger JC, et al. Plasmodium inui is not closely related to other quartan Plasmodium species. J. Parasitol. 84: 278-282, 1998. PubMed: 9576499
常見問題
答：公司提供兩種運輸方式供老師選擇，1、復蘇的活細胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復蘇的步驟，細胞成活率較高。2、凍存的細胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲，擇機復蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復蘇。
答：我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進行選擇！
答：不可以重復使用，一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細胞存活，控制生長速度，不可以用來做細胞培養(yǎng)使用。
答：細胞在鏡下發(fā)現(xiàn)圓形的白色的點點，一般情況下是為貼壁的細胞或脫落的細胞死亡后的產(chǎn)物，懸浮細胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團類的懸浮細胞可能會聚團出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細胞數(shù)量多一些，便于后期復蘇。購買原代細胞的客戶，要充分考慮該細胞的傳代次數(shù)限制，人源原代細胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實驗。
皖公網(wǎng)安備 34010402703761號
郭經(jīng)理：3554285629
