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產品詳情
ATCC? Number:30823?
Organism: Trypanosoma cruzi Chagas
Designations: Texas-Tulane
Isolation: 21-month-old Doberman, Brazos County, TX, 1976
Depositors: RG Yaeger
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1011: Diphasic blood agar mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cross References: Nucleotide (GenBank) : X56166 T.hegewischi gene for small subunit ribosomal RNA (16S-like rRNA).
Classification: KINGDOM: Protozoa
References: 5029: Williams GD, et al. Naturally occurring trypanosomiasis (Chagas' Disease) in dogs. J. Am. Vet. Med. Assoc. 171: 171-177, 1977. PubMed: 407202
Organism: Trypanosoma cruzi Chagas
Designations: Texas-Tulane
Isolation: 21-month-old Doberman, Brazos County, TX, 1976
Depositors: RG Yaeger
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1011: Diphasic blood agar mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cross References: Nucleotide (GenBank) : X56166 T.hegewischi gene for small subunit ribosomal RNA (16S-like rRNA).
Classification: KINGDOM: Protozoa
References: 5029: Williams GD, et al. Naturally occurring trypanosomiasis (Chagas' Disease) in dogs. J. Am. Vet. Med. Assoc. 171: 171-177, 1977. PubMed: 407202
常見問題
問：細胞的運輸方式有哪些？有什么區(qū)別？
答：公司提供兩種運輸方式供老師選擇，1、復蘇的活細胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復蘇的步驟，細胞成活率較高。2、凍存的細胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉入液氮長期存儲，擇機復蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復蘇。
問：為什么你們的細胞和其他公司的細胞培養(yǎng)條件不一樣？
答：我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案，根據客戶的意愿進行選擇！
問：培養(yǎng)瓶的培養(yǎng)液可以重復使用嗎？
答：不可以重復使用，一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細胞存活，控制生長速度，不可以用來做細胞培養(yǎng)使用。
問：培養(yǎng)細胞在鏡下觀察發(fā)現有一些白色的圓點是什么？
答：細胞在鏡下發(fā)現圓形的白色的點點，一般情況下是為貼壁的細胞或脫落的細胞死亡后的產物，懸浮細胞也會有這種現象，出現圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團類的懸浮細胞可能會聚團出現白色的亮斑，技術老師可以繼續(xù)培養(yǎng)并觀察。
問：剛買回來的細胞如何凍存留種呢？
答：一般情況下，我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細胞數量多一些，便于后期復蘇。購買原代細胞的客戶，要充分考慮該細胞的傳代次數限制，人源原代細胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數很少的原代細胞，不建議凍存，收到后調整狀態(tài)后即可安排實驗。
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