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別稱Bacteroides Vulgatus

貨號Delf-17420

規(guī)格 凍干/培養(yǎng)物

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產(chǎn)品詳情

Bacteria Collection: Bacteroides vulgatus
NCTC Number:NCTC 11154
Current Name:Bacteroides vulgatus
Original Strain Reference:ATCC 8482
Other Collection No:ATCC 8482
Previous Catalogue Name:Bacteroides vulgatus
Type Strain:Yes
Family:Bacteroidaceae
Hazard Group (ACDP):2
Release Restrictions:Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media:Fastidious anaerobe agar, 24-48 hours, 37°C, anaerobic
Conditions for growth on liquid media:bhi agar(bmrl formula),bhi broth(bmrl formula),37, strict anaerobe
Whole Genome Sequence:http://www.ebi.ac.uk/ena/data/view/ERS1273874
16S rRNA Gene Sequence:>gb|AF176693|ATCC8482|Bacteroides vulgatus 16S-23S rDNA intergenic spacer region.| ggagaggacgaaaga... >gb|AJ867050|ATCC 8482|Bacteroides vulgatus 16S rRNA gene, strain ATCC 8482.| caaactaggaccgag...
23S rRNA Gene Sequence:>gb|AF176693|ATCC8482|Bacteroides vulgatus 16S-23S rDNA intergenic spacer region.| ggagaggacgaaaga... >gb|AY155596|ATCC 8482|Bacteroides vulgatus 23S ribosomal RNA gene, partial sequence.| tagtcgaggagtcgg...
Bibliography:EGGERTH A H & GAGNON B H 1933 J BACT 25 389; CATO E P & JOHNSON J L 1976
Extended Bibliography:showhide Show bibliography
Ref #:25269
Author(s):Liu,C.;Song,Y.;McTeague,M.;Vu,A.W.;Wexler,H.;Finegold,S.M.
Journal:FEMS Microbiol Lett
Title:Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers
Volume:222
Page(s):9-16
Year:2003
Keyword(s):Bacteriological Techniques Bacteroides fragilis/*classification/*genetics/growth & development DNA Transposable Elements/genetics Phenotype Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics
Remarks:We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were first divided into three subgroups by multiplex PCR-G, followed by three multiplex PCR assays with three species-specific primer mixtures for identification to the species level. The primers were designed from nucleotide sequences of the 16S rRNA, the 16S-23S rRNA intergenic spacer region and part of the 23S rRNA gene. The established two-step multiplex PCR identification scheme was applied to the identification of 155 clinical isolates of the B. fragilis group that were previously identified to the species level by phenotypic tests. The new scheme was more accurate than phenotypic identification, which was accurate only 84.5% of the time. The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of the B. fragilis group species. This will permit more accurate assessment of the role of various B. fragilis group members in infections and of the degree of antimicrobial resistance in each of the group members.
URL:12757940
Data:Type strain / ATCC in 1977 / A.H. Eggerth in 1942 (Redeposited in 1954) / Eggerth, A. H. & Gagnon, B. H. (1933) J. Bact. 25, 389 / Moore, W. E. C. & Holdemann, L. V. (1973) Int. J. syst. Bact. 23, 69 / Cato, E. P. & Johnson, J. L. (1976) Int. J. syst. Bact. 26, 230
Accession Date:01/01/1977
History:ISOLATED BY EGGERTH A H,1942;REPLACEMENT IN 1954
Authority:Eggerth and Gagnon 1933 (AL)
Depositor:ATCC
Taxonomy:TaxLink: S508 (Bacteroides vulgatus Eggerth and Gagnon 1933) - Date of change: 5/02/2003
Biosafety Responsibility:It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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問：細(xì)胞的運輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲，擇機復(fù)蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復(fù)蘇。

問：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！

問：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。

問：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點點，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團類的懸浮細(xì)胞可能會聚團出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問：剛買回來的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實驗。