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產(chǎn)品詳情
NCIMB number
NCIMB 10664
Deposit type
Bacteria
Type strain
Yes
Nagoya restrictions
No
GMO
No
Taxon name
Clostridium haemolyticum
Depositor designation
McClung2027 Plummer29866
Preservation method
Lyophilised
Price band
A
Price(£)
130
Media
012
Gas regime
anaerobic
Incubation temperature (and/or range)
37°C
ACDP category
2
Hazard information
This species has been associated with pathogenic conditions in cattle, sheep and laboratory animals. Produced phospholipase C haemolyses red blood cells.
Additional hazard information
Clostridium haemolyticum is a recognized animal pathogen. It produces a variety of toxins including a phospholipase which causes a fatal haemolysis in cattle.
Cellular Shape
Rod
Cellular Motility
Yes
Gram stain
Gram Positive
Depositor Name
G.Hobbs
Depositor Company
Torry Research Station
Depositor Address
Aberdeen U.K.
Source
Bovine
Date of Accession
01/09/1971
Other collection IDs
ATCC9650 DSM5565
Yeast?
False
K12
No
References
Approved Lists of Bacterial Names, Int J Syst Bacteriol 30, 225-420, 1980 Phylogenetic basis for a taxonomic dissection of the genus Clostridium, FEMS Immunol Med Microbiol 24(3), 253-258, 1999 Phylogenetic positions of Clostridium novyi and Clostridium haemolyticum based on 16S rDNA sequences, Int J Syst Evol Microbiol 51(3), 901-904, 2001 Development and validation of a multiplex real-time PCR for detection of Clostridium chauvoei and Clostridium septicum, Mol Cell Probes 24(4), 204-210, 2010 Rapid identification and differentiation of pathogenic clostridia in gas gangrene by polymerase chain reaction based on the 16S-23S rDNA spacer region, Res Vet Sci 69(3), 289-294, 2000 Taxonomy of the Clostridia: ribosomal ribonucleic acid homologies among the species, J Gen Microbiol 88(2), 229-244, 1975 Phylogenetic analysis and PCR detection of Clostridium chauvoei, Clostridium haemolyticum, Clostridium novyi types A and B, and Clostridium septicum based on the flagellin gene, Vet Microbiol 86(3), 257-267, 2002
常見問題
答:公司提供兩種運輸方式供老師選擇,1、復蘇的活細胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復蘇的步驟,細胞成活率較高。2、凍存的細胞:采用干冰運輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉入液氮長期存儲,擇機復蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點是需要自己復蘇。
答:我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進行選擇!
答:不可以重復使用,一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多,通常在3-5%,以維持細胞存活,控制生長速度,不可以用來做細胞培養(yǎng)使用。
答:細胞在鏡下發(fā)現(xiàn)圓形的白色的點點,一般情況下是為貼壁的細胞或脫落的細胞死亡后的產(chǎn)物,懸浮細胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點。通常,白色的圓點是分散分布的,聚團類的懸浮細胞可能會聚團出現(xiàn)白色的亮斑,技術老師可以繼續(xù)培養(yǎng)并觀察。
答:一般情況下,我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細胞數(shù)量多一些,便于后期復蘇。購買原代細胞的客戶,要充分考慮該細胞的傳代次數(shù)限制,人源原代細胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實驗。



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