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Streptococcus Salivarius

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別稱Streptococcus Salivarius

貨號(hào)Delf-17546

規(guī)格 凍干粉/培養(yǎng)物

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產(chǎn)品詳情

Streptococcus salivarius Andrewes and Horder 1906
Taxonomy in NCBI database: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae.
5707T <-- DSM 20560 <-- ATCC 7073 <-- New York State Dept. Health, USA; 275 <-- J. C. Small.
Accessioned in 1986.
=ATCC 7073 =BCRC 17388 =CCM 4046 =CCUG 11878 =CCUG 17825 =CCUG 50207 =CECT 805 =CIP 102503 =DSM 20560 =HAMBI 1716 =LMG 11489 =NCFB 1779 =NCIMB 701779 =NCTC 8618.
Type strain [596].
Medium: 27, 70; Temperature: 37°C; Rehydration fluid: 663.

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Cell wall: [639].
Serology: Group K.
Phylogeny: 16S rRNA gene (AY188352, LC071828).
Genome sequence: CP009913, FRCU00000000, LR134274.
More information: Oral bacterial DNA-based saliva identification [12713].
NCBI Taxonomy ID: 1304.
Genomic DNA is available from RIKEN BRC-DNA Bank: JGD 08330.
Publication(s) using this strain [A04168, A06010, A07006, A07007, A07209, A08254, A09005, A10008, A10272, A10299, A10302, A10303, A11021, A11232, A12041, A12071, A12092, A12104, A12417, A12443, A13174, A14123, B14341, A14444, A15498, A15575, A17004, A17518, A18129, A18178, A18471, A19164, A19526, A20151].
Patent publication(s) using this strain [JP2008-206516A, JP2010-029130A, JP2011-001352A, JP2012-229191A].
Related information on delivery / use of the strain
Biosafety level1
Terms and conditionsNot applicable
Export control (1)No
Distribution control in Japan (2)No
Genetically modified microorganismNo
Technical information-
Additional information-
(1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
(2) in complying with the Plant Protection Law of Japan

Delivery category
DomesticA (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
OverseasA (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.

常見(jiàn)問(wèn)題

問(wèn)：細(xì)胞的運(yùn)輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過(guò)夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。

問(wèn)：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！

問(wèn)：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來(lái)做細(xì)胞培養(yǎng)使用。

問(wèn)：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類(lèi)的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問(wèn)：剛買(mǎi)回來(lái)的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買(mǎi)原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。