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小腸結(jié)腸炎耶爾森氏菌

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別稱(chēng)Yersinia Enterocolitica Subsp. Enterocolitica

貨號(hào)Delf-17656

規(guī)格 凍干粉/培養(yǎng)物

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產(chǎn)品詳情

Name: Yersinia enterocolitica subsp. enterocolitica (Schleifstein and Coleman 1939) Neubauer et al. 2000
DSM No.: 4780, Type strain dsm-4780 dsm4780 dsm 4780 Yersinia enterocolitica subsp. enterocolitica
Strain designation: 33114
Other collection no.
or WDCM no.: ATCC 9610,WDCM 00038,CIP 80.27,NCTC 12982
Serotype: described as serotype O:8 but DSMZ culture appears serologically rough
Isolated from: glanders-like infection of face
Country: country of origin unknown
Date of sampling: before 31.08.1988
Nagoya Protocol Restrictions: There are NO known Nagoya Protocol restrictions for this strain.
History: <- ATCC <- J.M. Coffey; 33114 (Bacterium enterocoliticum)
Genbank accession numbers: 16S rRNA gene: NR_041832
Cultivation conditions: Medium 215 , 30°C
or
Medium 535 , 30°C
or
Medium 693 , 30°C

rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates

Incubation time: 1-2 days

Complete DSMZ Media List
Summary and
additional information: <- ATCC <- J.M. Coffey; 33114 (Bacterium enterocoliticum). Glanders-like infection of face; country of origin unknown; sampling date: before 31.08.1988. Serotype described as serotype O:8 but DSMZ culture appears serologically rough. Type strain. Taxonomy/description (1300, 3497). Sequence accession no. 16S rRNA gene: NR_041832. CRMOX positive; Esc and Sal negative after 24 h; no plasmid found; autoagglutination test negative; rough colonies. Quality control strain according to DIN 58959-7 and 58959-9 (6924), Quality control strain according to DIN 58959-7 and 58959-9 (16730). Biotype 1. According to literature (7666) this specific strain is described as biotype 1A. However, American origin, 16S rRNA sequence and biochemical features (no esculin hydrolyses and no acid production from salicin within 48h) group the strain into biotype 1B. No plasmid detected at DSMZ. (Medium 215, 30°C or Medium 535, 30°C or Medium 693, 30°C). Rehydrate and grow lyophilized cells from the

常見(jiàn)問(wèn)題

問(wèn)：細(xì)胞的運(yùn)輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過(guò)夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。

問(wèn)：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶(hù)提供了替代培養(yǎng)方案，根據(jù)客戶(hù)的意愿進(jìn)行選擇！

問(wèn)：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來(lái)做細(xì)胞培養(yǎng)使用。

問(wèn)：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類(lèi)的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問(wèn)：剛買(mǎi)回來(lái)的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶(hù)收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買(mǎi)原代細(xì)胞的客戶(hù)，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。