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      產(chǎn)品詳情

      Organism:

      Lactobacillus plantarum (Orla-Jensen) Bergey et al. deposited as Lactobacillus arabinosus Fred et al.

      Designations:

      17-5 [BUCSAV 217, BUCSAV 449, Glaxo 664, ICPB 2080, NCDO 82, NCIB 6376, NCIB 8014, NCIB 8030]

      Depositor:

      E McCoy

      Biosafety Level:

      1             ATCC 8014 植物乳桿菌

      Shipped:

      freeze-dried

      Growth Conditions:

      ATCC medium416: Lactobacilli MRS broth
      Temperature: 37.0°C

      Permits/Forms:

      In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

      Cross References:

      Nucleotide (GenBank) : U97139 Lactobacillus plantarum 16S/23S ribosomal RNA large intergenic spacer region, tRNA-Ile and tRNA-Ala genes, complete sequence.

      Nucleotide GenBank: X62346 L.plantarum plasmid 8014-2 DNA from Bc1I to HincII site.

      Nucleotide GenBank: X99978 Lactobacillus plantarum citrulline biosynthetic gene cluster carAB operon and argC, J, B, D, F-ccl operonand usg and dsg partial sequences.

      Nucleotide GenBank: AF189765 Lactobacillus plantarum alpha-galactosidase melAgene, complete cds.

      Comments:

      each NCIB culture from a different depositor

      bacteriophage host

      accumulation of biotin [6553]

      D-biotin conversion and metabolism [34645] [58634] [10275]

      biotin transport and accumulation [11064] [34646]

      formation of acetylmethylcarbinol [7469]

      effect of pantothenate derivatives on growth and coenzyme-A synthesis [7301]

      sulfur nutrition [7482] [6175]

      nitrate reduction [6263]

      effect of oleic acid on free biotin uptake [7553]

      ribitol-5-phosphate dehydrogenase [7316]

      synthesis of ribitol teichoic acids [10745]

      energy-producing pathways [7540] [10737]

      DNA base composition 45.1 moles % GC[9315]

      Applications:

      assay of amino acids [6665]

      assay of biotin [92233]

      assay of calcium pantothenate [21607] [92233]

      assay of niacinamide nicotinamide [21610]

      assay of nicotinic acid niacin [21480] [21610] [5216]

      assay of pantothenic acid [21480]

      assay of tryptophan L-tryptophan

      metabolizes mevalonic acid [7531]

      produces lactic acid lactate [58392]

      quality control strain [92408]

      produces lactic acid from coconut water [58392]

      References:

      5216: AACC Niacin--microbiological method. St. Paul, MN, 1999

      6175: Arch. Biochem. 16: 357-360, 1948.

      6263: Costilow RN, Humphreys TW. Nitrate reduction by certain strains of Lactobacillus plantarum. Science 121: 168, 1955. PubMed: 13225760

      6553: Lichstein HC, Waller JR. Factors affecting the accumulation of biotin by Lactobacillus arabinosus. J. Bacteriol. 81: 65-69, 1961. PubMed:13761907

      6665: AOAC International Amino acids in vitamin preparations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 960.47.

      7301: Pierpoint WS, et al. The effect of some pantothenate derivatives on growth and coenzyme-A synthesis in bacteria. Biochem. J. 61: 190-197, 1955. PubMed: 13260197

      7316: . . Biochim. Biophys. Acta 67: 525-530, 1963.

      7469: Moat AG, Lichstein HC. Factors affecting the formation of acetylmethylcarbinol by lactobacillus arabinosus. J. Bacteriol. 66: 324-327, 1953. PubMed: 13096481

      常見問題
      問:細(xì)胞的運輸方式有哪些?有什么區(qū)別?

      答:公司提供兩種運輸方式供老師選擇,1、復(fù)蘇的活細(xì)胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟,細(xì)胞成活率較高。2、凍存的細(xì)胞:采用干冰運輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲,擇機復(fù)蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點是需要自己復(fù)蘇。

      問:為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣?

      答:我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進行選擇!

      問:培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎?

      答:不可以重復(fù)使用,一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多,通常在3-5%,以維持細(xì)胞存活,控制生長速度,不可以用來做細(xì)胞培養(yǎng)使用。

      問:培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點是什么?

      答:細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點點,一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物,懸浮細(xì)胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點。通常,白色的圓點是分散分布的,聚團類的懸浮細(xì)胞可能會聚團出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

      問:剛買回來的細(xì)胞如何凍存留種呢?

      答:一般情況下,我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細(xì)胞數(shù)量多一些,便于后期復(fù)蘇。購買原代細(xì)胞的客戶,要充分考慮該細(xì)胞的傳代次數(shù)限制,人源原代細(xì)胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細(xì)胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實驗。

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