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ATCC 10231 白色假絲酵母/白色念球菌

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貨號Delf-18126

規(guī)格 凍干粉/培養(yǎng)物

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產(chǎn)品詳情

Organism:Candida albicans （Robin） Berkhout, anamorphDesignations:3147 [CBS 6431, CCY 29-3-106, CIP 48.72, DSM 1386, IFO 1594, NCPF 3179, NCYC 1363, NIH 3147, VTT C-85161]Isolation:man with bronchomycosisDepositors:CW EmmonsHistory:ATCC <<-- CW Emmons<<-- WrightBiosafety Level:1Shipped:freeze-driedGrowth Conditions:ATCC medium 200: YM agar or YM brothTemperature: 25.0°C ATCC 10231 白色假絲酵母/白色念球菌Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Antigenic Properties:serotype A [19210]Applications:assay of amphotericin B fungizone [58605] assay of antimicrobial preservatives [11020] [21514] [21603] assay of haloprogin [58605] assay of nystatin fungicidin [58605] detection of [92841] media testing [11018] [11019] [21509] [21529] [21569] [21613] [92390] [92428] [92845] membrane filter testing [92565] preparatory test control [21613] produces D-arabinolactone oxidase [20456] produces DNA topoisomerase [17682] produces aspartic proteinases aspartyl proteinases [20538] produces estrogen-binding protein [1694] produces lanosterol synthase 2,3-oxidosqualene lanosterol cyclase [20455] produces phenethyl alcohol [594] produces polyamine oxidase [54167] [57063] produces tryptophol [594] quality control strain [92096] [92144] [92409] sterility testing [21604] [58442] [92277] [92306] testing [92305] [92307] [92402] [92403] testing fungicides [92443] [92784] [92789] [92802] [92824] [92831] [92836] [92837] produces farnesoic acid, an autoregulatory substance capable of regulating morphological transition [53041]Comments:This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified. For sterility testing, not more than five passages from the ATCC culture should be used. Growth and invasiveness in mouse [19748] Steroid interference with antifungal activity [19749] Cell wall hydrophobicity enhances corticosterone incorporation. [20319] Ultraviolet microscopy [20321] Calcification [20476] Morphology and physiology of strain sectors [20157] Use of impedance for preservative efficacy testing [1968] Fungitoxicity of alcohols and fatty acids [16096] Esterase activity [19297] Lipid composition [20072] Effect of antineoplastic drugs [19796]Related Products:genomic DNA: ATCC 10231D-5Subcollection:YeastsReferences:594: Lingappa BT, et al. Phenethyl alcohol and tryptophol: autoantibiotics produced by the fungus Candida albicans. Science 163: 192-194, 1969. PubMed: 57627681694: Skowronski R, Feldman D. Characterization of an estrogen-binding protein in the yeast Candida albicans. Endocrinology 124: 1965-1972, 1989. PubMed: 26474701968: Connolly P, et al. The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products. J. Appl. Bacteriol. 76: 68-74, 1994. PubMed: 81444072024: Klig LS, et al. Comparison of INO1 gene sequences and products in Candida albicans and Saccharomyces cerevisiae. Yeast 10: 789-800, 1994. PubMed: 79758964101: ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA11018: British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 200311019: British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 200311020: British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British

常見問題

問：細(xì)胞的運(yùn)輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲，擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。

問：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！

問：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。

問：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問：剛買回來的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗。