一鍵復(fù)制產(chǎn)品信息
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產(chǎn)品詳情
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Organism: |
Mycobacterium tuberculosis （Zopf） Lehmann and Neumann |
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Designations: |
H37Ra |
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Depositor: |
CL Larson |
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Biosafety Level: |
2 ATCC 25177 |
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Shipped: |
frozen |
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Growth Conditions: |
ATCC medium173: Middlebrook 7H10 agar with Middlebrook OADC enrichment Alternate medium 90: Lowenstein Jenson medium
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Permits/Forms: |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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Cross References: |
Nucleotide （GenBank） : AX110247 Sequence 980 from Patent WO0123604. Nucleotide （GenBank） : CP000611Mycobacterium tuberculosis H37Ra, complete genome. Nucleotide （GenBank） : U24555 Mycobacterium tuberculosis 23S rRNA, partial sequence. Nucleotide （GenBank） : U67924 class A beta-lactamase （blaA） gene sequence Nucleotide （GenBank） : X70901 M.tuberculosis mce gene. |
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Comments: |
Exhibits class A beta-lactamase activity [34276] [50662] Attenuated [7400] [10953] Genome sequenced strain |
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Applications: |
media testing [21508] [21529] susceptibility testing [92217] |
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Related Products: |
purified DNA: ATCC 25177D-5 |
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References: |
7400: McMurray DN, et al. Respiratory infection with attenuated Mycobacterium tuberculosis H37Ra in malnourished guinea pigs. Infect. Immun. 39: 793-799, 1983. PubMed: 6403460 10953: Alsaadi AI, Smith DW. The fate of virulent and attenuated Mycobacteria in guinea pigs infected by the respiratory route. Am. Rev. Respir. Dis. 107: 1041-1046, 1973. PubMed: 4197232 21508: European Pharmacopoeia Commission Mycobacterium tuberculosis. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.2, 1997 21529: CLSI Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA:Clinical and Laboratory Standards Institute;CLSI M22-A3. 32573: Li Z, et al. Rapid detection of Mycobacterium avium in stool samples from AIDS patients by immunomagnetic PCR. J. Clin. Microbiol. 34: 1903-1907, 1996. PubMed: 8818878 34276: Voladri RK, et al. Recombinant expression and characterization of the major B-lactamase of Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 42: 1375-1381, 1998. PubMed: 9624479 50662: Hackbarth CJ, et al. Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra. Antimicrob. Agents Chemother. 41: 1182-1185, 1997. PubMed: 9145897 92217: CLSI Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes; Approved Standard. Wayne, PA:Clinical and Laboratory Standards Institute;CLSI M24-A. |
常見問(wèn)題
答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過(guò)夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。
答：我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來(lái)做細(xì)胞培養(yǎng)使用。
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。
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