產(chǎn)品推薦
聯(lián)系方式
- 電話:400-1016-218
- 郵箱:355185756@qq.com
- 地址:安徽省合肥市高新區(qū)黃山路602號合肥國家大學科技園A401室
產(chǎn)品詳情
|
Deposited As |
Monilia tropicalis (Caslani) Caslani et Chalmers, anamorph |
|
Strain Designations |
[1909, ATCC 4563, ATCC 7349, CBS 94, CCRC 20520, DSY 59, IFO 1070, IFO 1400, JCM 1541, NRRL Y-12699, NRRL Y-12968, NRRL Y-607] |
|
Application |
Assay of chloro-8-quinolinol Bacterial resistance testing Degrades xylose D-xylose Fungus resistance testing adhesives Produces aspartic proteinases aspartyl proteinases Produces ethyl alcohol ethanol Produces xylitol Susceptibility disc testing Susceptibility testing Transformation host Produces xylitol from D-xylose Control strain for typing systems Produces ethanol from D-xylose Control strain to test oxygen availability in bioreactor in space laboratory Reference strain for Clinical and Laboratory Standards Institute(CLSI)-developed Antifungal Susceptibility Testing. |
|
Biosafety Level |
1 |
|
Product Format |
freeze-dried |
|
Storage Conditions |
Frozen: -80°C or colder |
|
Type Strain |
yes (type strain) |
|
Genotype |
ade2/ade2 acp/acp delta::lambda/delta::lambda |
|
Preceptrol? |
yes |
|
Genome Sequenced Strain |
Yes |
|
Comments |
Fluconazole-sensitive Utilizes phenol, catechol and 3- and 4-methylcatechols as sole carbon source Esterase activity MIC of 2-hydroxychalcone, 75 mcg/ml Adherence to lysophospholipids Genome sequencing strain (Genolevures Consortium, France; Wellcome Trust Sanger Institute, UK). For additional infromation, see CLSI M27-A2 (Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standards-Second Edition). Mitochondrial genome sequenced strain |
|
Medium |
ATCC? Medium 200: YM agar or YM broth ATCC ? Medium 1245: YEPD ATCC ? Medium 28: Emmons' modification of Sabouraud's agar |
|
Growth Conditions |
Temperature: 25°C Atmosphere: Typical aerobic |
常見問題
答:公司提供兩種運輸方式供老師選擇,1、復蘇的活細胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復蘇的步驟,細胞成活率較高。2、凍存的細胞:采用干冰運輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲,擇機復蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點是需要自己復蘇。
答:我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進行選擇!
答:不可以重復使用,一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多,通常在3-5%,以維持細胞存活,控制生長速度,不可以用來做細胞培養(yǎng)使用。
答:細胞在鏡下發(fā)現(xiàn)圓形的白色的點點,一般情況下是為貼壁的細胞或脫落的細胞死亡后的產(chǎn)物,懸浮細胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點。通常,白色的圓點是分散分布的,聚團類的懸浮細胞可能會聚團出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答:一般情況下,我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細胞數(shù)量多一些,便于后期復蘇。購買原代細胞的客戶,要充分考慮該細胞的傳代次數(shù)限制,人源原代細胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實驗。



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