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ATCC 14579菌種，蠟狀芽孢桿菌

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貨號Delf-18261

規(guī)格 凍干粉/培養(yǎng)物

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產(chǎn)品詳情

Bacillus cereus Frankland and Frankland (ATCC? 14579?)

Strain Designations	[BCRC 10603, CCM 2010, CCUG 7414, CIP 66.24, DSM 31, HAMBI 1887, HAMBI 1905, IAM 12605, JCM 2152, LMG 6923, NBRC 15305, NCCB 75008, NCIMB 9373, NCTC 2599, NRRL B-3711, VKM B-504]
Application	Produces restriction endonuclease Bce14579I Food testing
Type Strain	yes
Biosafety Level	1
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol?	yes
Genome Sequenced Strain	Bacillus cereus ATCC 14579, complete genome.
Type Strain	yes ATCC 14579菌種，蠟狀芽孢桿菌
Comments	Genome sequenced strain
Medium	ATCC? Medium 3: Nutrient agar or nutrient broth
Growth Conditions	Temperature: 30°C Atmosphere: Aerobic
Name of Depositor	RE Gordon
Special Collection	ATCC
References	Lawrence TS, Ford WW. Studies on aerobic spore-bearing non-pathogenic bacteria. Spore-bearing bacteria in milk. J. Bacteriol. 1: 277-320, 1916. J. Gen. Microbiol. 34: 271, 1964. Frankland GC, Frankland PF. Studies on some new micro-organisms from air. Philos. Trans. R. Soc. Lond. B Biol. Sci. 173: 257-287, 1887. Shibata T, et al. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains. J. Bacteriol. 128: 473-476, 1976. PubMed: 824277 Leonard C, et al. Integrated physical and genetic mapping of Bacillus cereus and other gram-positive bacteria based on IS231A transposition vectors. Infect. Immun. 66: 2163-2169, 1998. PubMed: 9573103 Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885 Identification of Presumptive Isolates of Bacillus anthracis. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 2004.04. Identification of Bacillus anthracis from Culture -- Gas Chromatographic Analysis of Fatty Acid Methyl Esters (FAMEs). Gaithersburg, MD
Cross References	Nucleotide (GenBank) : X98626 B.cereus SNF2 gene, partial. Nucleotide (GenBank) : Y10927 B.cereus bct1 and bcglr genes. Nucleotide (GenBank) : AX110270 Sequence 1003 from Patent WO0123604. Nucleotide (GenBank) : AX111054 Sequence 1787 from Patent WO0123604. Nucleotide (GenBank) : AX111073 Sequence 1806 from Patent WO0123604. Nucleotide (GenBank) : AX111085 Sequence 1818 from Patent WO0123604. Nucleotide (GenBank) : L48555 Bacillus cereus (library ATCC 14579) vrrA gene. Nucleotide (GenBank) : L37572 Bacillus cereus GyrB-GyrA intergenic spacer, DNA sequence. Nucleotide (GenBank) : L37576 Bacillus cereus 16S-23S rRNA intergenic spacer, DNA sequence. Nucleotide (GenBank) : AJ304815 Bacillus cereus partial clo1 gene for putative cereolysin O. Nucleotide (GenBank) : AJ304816 Bacillus cereus partial clo2 gene for putative cereolysin O. Nucleotide (GenBank) : AJ007952 Bacillus cereus celR, bct499b, chrA, ydjC and celF genes, partial. Nucleotide (GenBank) : AJ272333 Bacillus cereus cheA gene for histidine kinase, strain ATCC 14579. Nucleotide (GenBank) : AJ007794 Bacillus cereus hblC, hblD, hblA, hblB, kin1 and gerX genes, partial. Nucleotide (GenBank) : AJ272330 Bacillus cereus fliY gene for component of flagellar switch complex, strain ATCC 14579. Nucleotide (GenBank) : AJ007795 Bacillus cereus yndD, yndE, yndF, proC, yfnA, orf6, sspC, trrA, and hblC genes, partial. Nucleotide (GenBank) : AJ243712 Bacillus cereus yvfR (partial), yvfS, yvfT, yvfU, nprB, plcR, ykoW, ywjD and ywjE genes and ORF2. 79 21.2 kb chromosomal locus containing genes encoding enterotoxic hemolysin BL and surrounding regions; yndD (partial), yndE, yndF, proC, yfnA, sspC, trp1, hblC, hblD, hblA, hblB, shk1 and ger (partial) genes. Complete Genome (GenBank) : AE016877 Bacillus cereus ATCC 14579, complete genome.Nucleotide (GenBank) : AJ237785 Bacillus cereus ATCC 145

常見問題

問：細胞的運輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運輸方式供老師選擇，1、復蘇的活細胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復蘇的步驟，細胞成活率較高。2、凍存的細胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲，擇機復蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復蘇。

問：為什么你們的細胞和其他公司的細胞培養(yǎng)條件不一樣？

答：我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進行選擇！

問：培養(yǎng)瓶的培養(yǎng)液可以重復使用嗎？

答：不可以重復使用，一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細胞存活，控制生長速度，不可以用來做細胞培養(yǎng)使用。

問：培養(yǎng)細胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點是什么？

答：細胞在鏡下發(fā)現(xiàn)圓形的白色的點點，一般情況下是為貼壁的細胞或脫落的細胞死亡后的產(chǎn)物，懸浮細胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團類的懸浮細胞可能會聚團出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問：剛買回來的細胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細胞數(shù)量多一些，便于后期復蘇。購買原代細胞的客戶，要充分考慮該細胞的傳代次數(shù)限制，人源原代細胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實驗。