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ATCC 25374菌種，海洋鹽單胞菌

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貨號(hào)Delf-18289

規(guī)格 凍干粉/培養(yǎng)物

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產(chǎn)品詳情

Cobetia marina (Cobet et al.) Arahal et al. (ATCC? 25374?)

Deposited As	Arthrobacter marinus Cobet et al.
Strain Designations	DSM 4741 [219, CECT 4278, CIP 104765, LMG 2217, NCIMB 1877]
Isolation	Seawater, Woods Hole, MA
Type Strain	yes Ref, Ref, Ref, Ref, Ref, Ref
Biosafety Level	1
Product Format	freeze-dried

Preceptrol?	no
Type Strain	yes Ref, Ref, Ref, Ref, Ref, Ref

Medium	ATCC? Medium 2: Marine agar 2216 or marine broth 2216
Growth Conditions	Temperature: 26.0°C

Name of Depositor	AB Cobet ATCC 25374菌種，海洋鹽單胞菌
Chain of Custody	ATCC <<--AB Cobet<<--G. Jones <<--- C. Wirsen
Isolation	Seawater, Woods Hole, MA
References	Cobet AB, et al. The effect of nickel on a marine bacterium, Arthrobacter marinus sp. nov.. J. Gen. Microbiol. 62: 165, 1970. Baumann L, et al. Description of Deleya gen. nov. created to accommodate the marine species Alcaligenes aestus, A. pacificus, A. cupidus, A. venustus, and Pseudomonas marina. Int. J. Syst. Bacteriol. 33: 793-802, 1983. Baumann L, et al. Taxonomy of aerobic marine eubacteria. J. Bacteriol. 110: 402-429, 1972. PubMed: 4552999 Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. Dobson SJ, Franzmann PD. Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int. J. Syst. Bacteriol. 46: 550-558, 1996. Validation list no. 88. Int. J. Syst. Evol. Microbiol. 52: 1915-1916, 2002. Arahal DR, et al. Proposal of Cobetia marina gen. nov., comb. nov., within the family Halomonadaceae, to include the species Halomonas marina. Syst. Appl. Microbiol. 25: 207-211, 2002. PubMed: 12353874 name changed from Halomonas marina to Cobetia marina per routing slip of 1/30/2003 from Shirley Landon, Syst. Appl. Microbiol. 25: 207-211, 2002; IJSEM 52: 1915-1916, 2002. Cobet AB, et al. The effect of nickel on a marine bacterium, Arthrobacter marinus sp. nov.. J. Gen. Microbiol. 62: 165, 1970. Baumann L, et al. Description of Deleya gen. nov. created to accommodate the marine species Alcaligenes aestus, A. pacificus, A. cupidus, A. venustus, and Pseudomonas marina. Int. J. Syst. Bacteriol. 33: 793-802, 1983. Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. Dobson SJ, Franzmann PD. Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int. J. Syst. Bacteriol. 46: 550-558, 1996. Validation list no. 88. Int. J. Syst. Evol. Microbiol. 52: 1915-1916, 2002. Arahal DR, et al. Proposal of Cobetia marina gen. nov., comb. nov., within the family Halomonadaceae, to include the species Halomonas marina. Syst. Appl. Microbiol. 25: 207-211, 2002. PubMed: 12353874 Cobet AB, et al. The effect of nickel on a marine bacterium, Arthrobacter marinus sp. nov.. J. Gen. Microbiol. 62: 165, 1970. Baumann L, et al. Description of Deleya gen. nov. created to accommodate the marine species Alcaligenes aestus, A. pacificus, A. cupidus, A. venustus, and Pseudomonas marina. Int. J. Syst. Bacteriol. 33: 793-802, 1983. Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. Dobson SJ, Franzmann PD. Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int. J. Syst. Bacteriol. 46: 550-558, 1996. Validation list no. 88. Int. J. Syst. Evol. Microbiol. 52: 1915-1916, 2002. Arahal DR, et al. Proposal of Cobetia marina gen. nov., comb. nov., within the family Halomonadaceae, to include the species Halomonas marina. Syst. Appl. Microbiol. 25: 207-211, 2002. PubMed: 12353874
Cross References	Nucleotide (GenBank) : M93354 16S rRNA sequence

常見問題

問：細(xì)胞的運(yùn)輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。

問：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！

問：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。

問：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問：剛買回來的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。