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產(chǎn)品詳情
Lactobacillus casei (Orla-Jensen) Hansen and Lessel (ATCC? 393?)
|
Deposited As |
Lactobacillus casei subsp. casei (Orla-Jensen) Hansen and Lessel |
|
Strain Designations |
03 [7, IAM 12473, Orland L-323, R.P. Tittsler 303] |
|
Application |
Produces acetoin acetylmethylcarbinol Produces diacetyl Quality control strain |
|
Isolation |
Dairy products |
|
Type Strain |
yes |
|
Biosafety Level |
1 |
|
Product Format |
freeze-dried |
|
Storage Conditions |
Frozen: -80°C or colder |
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Preceptrol? |
yes |
|
Type Strain |
yes |
|
Comments |
Alpha-acetolactate biosynthesis |
|
Medium |
ATCC? Medium 416: Lactobacilli MRS Agar/Broth ATCC ? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
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Growth Conditions |
Temperature: 37°C Atmosphere: 5% CO2 |
|
Name of Depositor |
GJ Hucker |
|
Chain of Custody |
ATCC <-- GJ Hucker <-- S Orla-Jensen 7 (Streptobacterium casei) |
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Isolation |
Dairy products |
|
References ATCC 393菌種， 干酪乳桿菌 |
Hansen PA, Lessel EF. Lactobacillus casei (Orla-Jensen) comb. nov.. Int. J. Syst. Bacteriol. 21: 69-71, 1971. Collins MD, et al. Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans, and lactobacillus rhamnosus sp. nov., comb. nov.. Int. J. Syst. Bacteriol. 39: 105-108, 1989.
Wayne LG. Actions of the Judicial Commission of the International Committee on Systematic Bacteriology on requests for opinions published between January 1985 and July 1993. Int. J. Syst. Bacteriol. 44: 177-178, 1994. Alpert CA, Siebers U. The lac operon of Lactobacillus casei contains lacT, a gene coding for a protein of the Bg1G family of transcriptional antiterminators. J. Bacteriol. 179: 1555-1562, 1997. PubMed: 9045813 Auvray F, et al. Plasmid integration in a wide range of bacteria mediated by the integrase of Lactobacillus delbrueckii bacteriophage mv4. J. Bacteriol. 179: 1837-1845, 1997. PubMed:9068626 Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. Barnes AL, Keenan TW. Biosynthesis of alpha-acetolacetate and its converison of diacetyl and acetion in cell-free extracts of Lactobacillus casei. Can. J. Microbiol. 18: 479-485, 1972. PubMed: 4623970 AN MicroPlate QC Set. Biolog. ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions) Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM. |
常見(jiàn)問(wèn)題
答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過(guò)夜，第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。
答：我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長(zhǎng)速度，不可以用來(lái)做細(xì)胞培養(yǎng)使用。
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購(gòu)買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。
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