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產(chǎn)品詳情
Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC? 19433?)
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Deposited As |
Streptococcus faecalis Andrewes and Horder |
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Strain Designations |
NCTC 775 [DSM 20478, JCM 8726, NCDO 581, Tissier] |
|
Application |
Control Culture Media testing Quality control strain Reference material Control strain for Group D Quality control strain for Difco products Water testing |
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Type Strain |
yes |
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Biosafety Level |
2 |
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Product Format |
freeze-dried |
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Storage Conditions |
Frozen: -80°C or colder |
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Preceptrol? |
yes |
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Type Strain |
yes |
|
Comments |
Taxonomy |
|
Medium |
ATCC? Medium 44: Brain Heart Infusion Agar/Broth |
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Growth Conditions |
Temperature: 37°C Atmosphere: Aerobic |
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Name of Depositor |
NCTC |
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Chain of Custody |
ATCC <-- NCTC <-- NIRD |
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References |
Shattock PM. The streptococci of Group D; the serological grouping of Streptococcus boivis and observations on serologically refractory group D strains. J. Gen. Microbiol. 3: 80-92, 1949. Jones D, Shattock PM. The location of the group antigen of group D Streptococcus. J. Gen. Microbiol. 23: 335-343, 1960. PubMed:13790502 Schleifer KH, Kilpper-Balz R. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov.. Int. J. Syst. Bacteriol. 34: 31-34, 1984. Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. BD Crystal Gram-Positive QC Set. BD Becton Dickinson; Crystal Water microbiology. Method 8: Faecal streptococci--Estimation of the most probable number(MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.8-1995. Water microbiology. Method 9: Faecal streptococci -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.9-1995. Escherichia coli in Water by Membrane Filtration using membrane-Thermotolerant Escherichia coli Agar (mTEC). Washington, DC:Environmental Protection Agency;EPA EPA Method 1103.1, 2005 Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1106.1, 2005 Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-B-D-Glucoside Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1600, 2005 Escherichia coli in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1603, 2005 Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenation using Lauryl Tryptose Broth (LTB) and EC Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1680, 2005 Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003. Applied Biosystems, MicroSeq PHL Panel. Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003. BD Crystal Rapid Gram-Positive QC Set. BD Becton Dickinson. ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions) Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM. |
常見(jiàn)問(wèn)題
答:公司提供兩種運(yùn)輸方式供老師選擇,1、復(fù)蘇的活細(xì)胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟,細(xì)胞成活率較高。2、凍存的細(xì)胞:采用干冰運(yùn)輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過(guò)夜,第二天轉(zhuǎn)入液氮長(zhǎng)期存儲(chǔ),擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快,一般一兩天即可收到,缺點(diǎn)是需要自己復(fù)蘇。
答:我公司提供的細(xì)胞大部分都參考資源庫(kù)的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶(hù)提供了替代培養(yǎng)方案,根據(jù)客戶(hù)的意愿進(jìn)行選擇!
答:不可以重復(fù)使用,一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多,通常在3-5%,以維持細(xì)胞存活,控制生長(zhǎng)速度,不可以用來(lái)做細(xì)胞培養(yǎng)使用。
答:細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn),一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物,懸浮細(xì)胞也會(huì)有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常,白色的圓點(diǎn)是分散分布的,聚團(tuán)類(lèi)的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答:一般情況下,我公司建議客戶(hù)收到細(xì)胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細(xì)胞數(shù)量多一些,便于后期復(fù)蘇。購(gòu)買(mǎi)原代細(xì)胞的客戶(hù),要充分考慮該細(xì)胞的傳代次數(shù)限制,人源原代細(xì)胞大概可以傳7代左右,鼠源的可以傳3代左右,對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。



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