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產(chǎn)品詳情
Lactobacillus plantarum subsp. plantarum (Orla-Jensen) Bergey et al. (ATCC? 14917?)
|
Deposited As |
Lactobacillus plantarum (Orla-Jensen) Bergey et al. |
|
Strain Designations |
Lp 39 [IAM 12477] |
|
Application |
Quality control strain |
|
Isolation |
Pickled cabbage |
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Type Strain |
yes |
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Biosafety Level |
1 |
|
Product Format |
freeze-dried |
|
Storage Conditions |
Frozen: -80°C or colder |
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Preceptrol? |
no |
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Genome Sequenced Strain |
Yes |
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Type Strain |
yes |
|
Comments |
This strain has been sequenced as a reference genome for the NIH Human Microbiome Project. |
|
Medium |
ATCC? Medium 416: Lactobacilli MRS Agar/Broth |
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Growth Conditions |
Temperature: 37°C Atmosphere: 5% CO2 |
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Name of Depositor |
PA Hansen |
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Chain of Custody |
ATCC <-- PA Hansen <-- Roy Techn. Coll., Copenhagen <-- S. Orla-Jensen (Streptobacterium plantarum) |
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Isolation |
Pickled cabbage |
|
References |
. . Mem. Acad. R. Sci. Denmark, Sect. Sci. Biol. 2: 1-145, 1943. . . Mem. Acad. R. Sci. Denmark, Sect. Sci. 8 Ser. 5: 81-197, 1919. . Type strains of Lactobacillus species. Rockville, MD: ATCC; 1968. Mayo B, et al. Cloning and characterization of cspL and cspP, two cold-inducible genes from Lactobacillus plantarum. J. Bacteriol. 179: 3039-3042, 1997. PubMed: 9139925 Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions) Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM. |
|
Cross References |
Nucleotide (GenBank) : X12886 Lactobacillus plantarum 5S ribosomal RNA. Nucleotide (GenBank) : AJ286119 Lactobacillus plantarum partial recA gene for RecA protein. Nucleotide (GenBank) : U97133 Lactobacillus plantarum 16S/23S ribosomal RNA small intergenic spacer region, complete sequence. Nucleotide (GenBank) : AF080101 Lactobacillus plantarum 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer region, complete sequence; and 23S ribosomal RNA gene, partial sequence. Nucleotide (GenBank) : ACGZ01000000 Lactobacillus plantarum subsp. plantarum ATCC 14917, whole genome shotgun sequencing project |
常見問題
答:公司提供兩種運(yùn)輸方式供老師選擇,1、復(fù)蘇的活細(xì)胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟,細(xì)胞成活率較高。2、凍存的細(xì)胞:采用干冰運(yùn)輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲,擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快,一般一兩天即可收到,缺點是需要自己復(fù)蘇。
答:我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進(jìn)行選擇!
答:不可以重復(fù)使用,一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多,通常在3-5%,以維持細(xì)胞存活,控制生長速度,不可以用來做細(xì)胞培養(yǎng)使用。
答:細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點點,一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物,懸浮細(xì)胞也會有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點。通常,白色的圓點是分散分布的,聚團(tuán)類的懸浮細(xì)胞可能會聚團(tuán)出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答:一般情況下,我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細(xì)胞數(shù)量多一些,便于后期復(fù)蘇。購買原代細(xì)胞的客戶,要充分考慮該細(xì)胞的傳代次數(shù)限制,人源原代細(xì)胞大概可以傳7代左右,鼠源的可以傳3代左右,對于一些能傳代次數(shù)很少的原代細(xì)胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實驗。



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