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ATCC 12228 表皮葡萄球菌

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貨號Delf-18110

規(guī)格 凍干粉/培養(yǎng)物

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產(chǎn)品詳情

Preceptrol? Culture
Organism:	Staphylococcus epidermidis （Winslow and Winslow） Evans
Designations:	FDA strain PCI 1200
Depositor:	FDA
Biosafety Level:	1 ATCC 12228 表皮葡萄球菌
Shipped:	freeze-dried
Growth Conditions:	ATCC medium3: Nutrient agar or nutrient broth Temperature: 37.0°C Atmosphere: Aerobic
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cross References:	Nucleotide （GenBank） : AE015929Staphylococcus epidermidis ATCC 12228, complete genome. Nucleotide （GenBank） : U39771 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region. Nucleotide （GenBank） : U90020 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region. Nucleotide （GenBank） : U90018 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region. Nucleotide （GenBank） : U90019 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region. Nucleotide （GenBank） : U90021 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.
Comments:	Genome sequenced strain
Applications:	assay of [11016] [92287] [92303] assay of gentamicins gentamicin [21512] [21605] assay of neomycin [21605] [21742] [92286] assay of netilmycin [21605] assay of novobiocin [21605] [21742] assay of oleandomycin assay of paromomycin aminosidine, catenulin [21605] assay of sisomicin [21605] inhibition testing [92580] media testing [21529] [92390] [92845] quality control strain [92096] [92128] [92409] sterility testing susceptibility disc testing neomycin susceptibility disc testing vancomycin testing
Related Products:	purified DNA:ATCC 12228D-5
References:	11016: British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003 21512: European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 21529: CLSI Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA:Clinical and Laboratory Standards Institute;CLSI M22-A3. 21605: U.S. Pharmacopeia General Chapters;<81>ANTIBIOTICS-MICROBIAL ASSAYS. Rockville, MD:U.S. Pharmacopeia;USP USP28-NF23, 2005 21742: AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23. 92096: Applied Biosystems MicroSeq Pharmaceutical Panel #1. :Applied Biosystems;MicroSeq 92128: BD Crystal Gram-Positive. :BD Becton Dickinson;Crystal 92286: Neomycin in Feeds -- Stahl Microbiological Agar Diffusion Assay. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.02. 92287: Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10. 92303: Microbial assay for antibiotics. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.34. 92390: Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva （Switzerland）:International Organization for Standardization/ANSI;ISO ISO 11133-2:2003. 92409: Applied Biosystems MicroSeq PHL Panel #1. :Applied Biosystems;MicroSeq 92580: Standard Test Method for Evaluation of Antimicrobial Formulations by the Agar Patch Technique. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1882-05. 92845: Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

常見問題

問：細胞的運輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運輸方式供老師選擇，1、復(fù)蘇的活細胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟，細胞成活率較高。2、凍存的細胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲，擇機復(fù)蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復(fù)蘇。

問：為什么你們的細胞和其他公司的細胞培養(yǎng)條件不一樣？

答：我公司提供的細胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進行選擇！

問：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細胞都需要達到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細胞存活，控制生長速度，不可以用來做細胞培養(yǎng)使用。

問：培養(yǎng)細胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點是什么？

答：細胞在鏡下發(fā)現(xiàn)圓形的白色的點點，一般情況下是為貼壁的細胞或脫落的細胞死亡后的產(chǎn)物，懸浮細胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團類的懸浮細胞可能會聚團出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問：剛買回來的細胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細胞的客戶，要充分考慮該細胞的傳代次數(shù)限制，人源原代細胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實驗。