一鍵復(fù)制產(chǎn)品信息
產(chǎn)品推薦
聯(lián)系方式
- 電話：400-1016-218
- 郵箱：355185756@qq.com
- 地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學(xué)科技園A401室
產(chǎn)品詳情
Lactobacillus pentosus (Fred et al.) Zanoni et al. (ATCC? 8041?)
|
Strain Designations |
124-2 [DSM 20314, NCDO 363, NCIB 8026] |
|
Application |
Degrades sawdust Ferments hexoses Ferments pentoses Produces acetic acid Produces lactic acid lactate Fermentation of wood sugar remaining after alcoholic fermentation Production of acetic and lactic acids from mill sawdust Production of lactic acid by fermentation of sulfite waste liquor |
|
Type Strain |
yes Ref |
|
Biosafety Level |
1 |
|
Product Format |
freeze-dried |
|
Preceptrol? |
no |
|
Type Strain |
yes Ref |
|
Comments |
Requirement for pantothenic and nicotinic acids Synthesis of riboflavin Purine and pyrimidine bases as growth substances Sulfanilamide antagonists as growth factors Amino-acid requirements Vitamin requirements Nutritional requirements Metabolism of biotin and oxybiotin Electron transport to oxygen Numerical taxonomy ATCC 8041菌種， 戊糖乳桿菌 |
|
Medium |
ATCC? Medium 416: Lactobacilli MRS Agar/Broth |
|
Growth Conditions |
Temperature: 37.0°C |
|
Name of Depositor |
EE Snell |
|
References |
Proc. Natl. Acad. Sci. USA 27: 1-7, 1941. . . J. Am. Chem. Soc. 60: 2825, 1938. . . J. Biol. Chem. 168: 23-31, 1947. . . J. Bacteriol. 38: 293-308, 1939. . . J. Bacteriol. 55: 683-692, 1948. . . J. Bacteriol. 55: 693-703, 1948. Fred EB, et al. The characteristics of certain pentose destroying bacteria, especially as concerns their action on arabinose. J. Biol. Chem. 48: 385-412, 1921. . . J. Biol. Chem. 53: 111-123, 1922. . . Enzymologia 6: 186-193, 1939. Seyfried PL. An approach to the classification of lactobacilli using computer-aided numerical analysis. Can. J. Microbiol. 14: 313-318, 1968. PubMed: 5646833 Zanoni P, et al. Lactobacillus pentosus (Fred, Peterson, and Anderson) sp. nov., nom rev. Int. J. Syst. Bacteriol. 37: 339-341, 1987. Strittmatter CF. Electron transport to oxygen in lactobacilli. J. Biol. Chem. 234: 2789-2793, 1959. PubMed: 13856390 Ind. Eng. Chem., Ind. Ed. 19: 1162-1165, 1927. . . Arch. Biochem. 2: 93-101.. Allgeier RJ, et al. Production of acetic and lactic acids from mill sawdust. Ind. Eng. Chem. 21: 1039-1042, 1929. Leonard RH, et al. Lactic acid from fermentation of sulfide waste liquor. Ind. Eng. Chem. 40: 57-67, 1948. Dunn MS, et al. The amino acid requirements of twenty-three lactic acid bacteria. J. Biol. Chem. 168: 1-22, 1947. Zanoni P, et al. Lactobacillus pentosus (Fred, Peterson, and Anderson) sp. nov., nom rev. Int. J. Syst. Bacteriol. 37: 339-341, 1987. Zanoni P, et al. Lactobacillus pentosus (Fred, Peterson, and Anderson) sp. nov., nom rev. Int. J. Syst. Bacteriol. 37: 339-341, 1987. |
|
Cross References |
Nucleotide (GenBank) : U97134 Lactobacillus pentosus 16S/23S ribosomal RNA small intergenic spacer region, complete sequence. Nucleotide (GenBank) : U97140 Lactobacillus pentosus 16S/23S ribosomal RNA large intergenic spacer region, tRNA-Ile and tRNA-Ala genes, complete sequence. |
常見問題
答：公司提供兩種運輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲，擇機復(fù)蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復(fù)蘇。
答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進行選擇！
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點點，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團類的懸浮細(xì)胞可能會聚團出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實驗。
皖公網(wǎng)安備 34010402703761號
夏經(jīng)理：341412994
