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Ax-3

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別稱Dictyostelium Discoideum

貨號Delf-17230

規(guī)格 Freeze-Dried

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產(chǎn)品詳情

Dictyostelium discoideum Raper 拉丁名

ATCC 28368 編號

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.

Product category Fungi

Strain designation 別名 Ax-3

Type strain 模式菌株 No

Product format 提供形式 Freeze-dried

Storage conditions 保藏溫度 2°C to 8°C

Specific applications produces calmodulin transformation host hydrolysis of a phosphonate ester

Preceptrol No

Comments Actin binding to plasma membrane Thymidine kinase activity Part of the Eumycetozoan Project This strain may grow in axenic medium or with Klebsiella aerogens, but ATCC maintains this strain with a bacterial food source.

Medium 培養(yǎng)基 ATCC Medium 617: Bg-11 for marine blue-green algae

ATCC Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)

Temperature 培養(yǎng)溫度 20-25°C

Atmosphere 需氧情況 Aerobic

Handling procedure For freeze-dry (lyophilized) ampoules: Open an ampoule according to enclosed instructions.

From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.

Aseptically transfer the suspension back into the test tube of sterile distilled water. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi. Mix the suspension well. Use 1.0 mL to inoculate recommended freshly-made and wetted solid medium and freshly-made liquid medium.

Agar plates should be overlaid with 2 mL of a liquid medium, either #671 or #1034 (with 10% fetal bovine serum included).

Include a control that receives no inoculum. Incubate the inoculum at the propagation conditions recommended. Inspect for growth of the inoculum/strain regularly.

The sign of viability is noticeable typically after 2-3 days of incubation.

However, the time necessary for significant growth will vary. Subculture from stationary liquid cultures after one week.

Sequenced data No DNA sequencing was performed in house on this product.

Deposited as Dictyostelium discoideum Raper Depositors WF Loomis

Chain of custody ATCC <-- WF Loomis

Special collection Eumycetozoan Project

Cross references 參考文獻(xiàn) GenBank D12883 gene coding for gp138 GenBank D12884 gene coding for gp138

常見問題

問：細(xì)胞的運輸方式有哪些？有什么區(qū)別？

答：公司提供兩種運輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲，擇機(jī)復(fù)蘇。優(yōu)勢是發(fā)貨快，一般一兩天即可收到，缺點是需要自己復(fù)蘇。

問：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？

答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！

問：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？

答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會比正常培養(yǎng)時所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。

問：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點是什么？

答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點點，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點。通常，白色的圓點是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

問：剛買回來的細(xì)胞如何凍存留種呢？

答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實驗。