ATCC? Number:50661?
Organism: Acanthamoeba sp. 4
Designations: 66-1A-MX
Isolation: Apalachicola River, Florida, 1981
Depositors: TK Sawyer
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 711: PYBATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Protocol: ATCCNO: 50655 SPEC: The strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Remove the frozen ampule from the dry ice and transfer directly to a 35C water bath. After thawing the ampule, transfer the contents to the surface of an ATCCmedium 997 or 711 agar plate (20 x 100 mm). Spread the material evenly over the surface of the plate with a sterile spread bar. The food bacterium, Enterobacter aerogenes ATCC13048, is present in the thawed material. Wrap the plate with time tape or parafilm and incubate upright at 25C. Many trophozoites should be visible within 2-3 days. Transfer the culture every 28 days as follows: Aseptically remove a small block of agar (approximately 5 mm square) containing cysts and, at the edge of an ATCCmedium 997 or 711 agar plate containing a lawn of Enterobacter aerogenes ATCC13048, invert the block onto the surface. Wrap the new plate and incubate as above. The trophozoites will migrate away from the agar block to the opposite edge of the plate. Continue to subculture as above.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: phylogeny [24028]
Classification: KINGDOM: Protozoa
References: 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681