腸沙門氏菌腸炎亞種
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一鍵復(fù)制產(chǎn)品信息
別稱Salmonella Enterica Subsp. Enterica
貨號(hào)Delf-17248
規(guī)格
產(chǎn)品推薦
聯(lián)系方式
- 電話：400-1016-218
- 郵箱：355185756@qq.com
- 地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國家大學(xué)科技園A401室
產(chǎn)品詳情
Salmonella enterica subsp. enterica (ex Kauffmann and Edwards) Le Minor and Popoff serovar Typhimurium 拉丁名
ATCC 14028 統(tǒng)一編號(hào)
Strain Designations 菌株別名 : CDC 6516-60 /
Type Strain 模式菌株 : no /
Biosafety Level 生物安全等級(jí) : 2
Deposited As Salmonella typhimurium (Loeffler) Castellani and Chalmers
Application 用途 Control Culture Media testing
Preparatory test control
Enteric Research
Emerging infectious disease research
Pharmaceutical and Personal Care
Water testing
Isolation 分離基物 Tissue, animal - pools of heart and liver from 4-week-old chickens
Type Strain 模式菌株 no
Biosafety Level 生物安全等級(jí) 2
Antigenic Properties I 4,5,12:i:1,2 (Determined by depositor)
Product Format 提供形式 freeze-dried
Storage Conditions 保藏方法
Frozen（冷凍物）: -80℃ or colder
Freeze-Dried（凍干物）: 2℃ to 8℃
Live Culture（活物）: See Propagation Section
Preceptrol? yes
Type Strain 模式菌株 no
Antigenic Properties I 4,5,12:i:1,2 (Determined by depositor)
Comments 注釋 This strain is recommended by ATCC for use in the tests described in U.S. Pharmacopeia, 23rd rev.,, p. 2180, 1995, where only the taxon is specified. The depositor states that this strain possesses these antigenic properties: 4,5,12:i:1,2. Two colony types can be distinguished on sheep blood agar. One colony type reacts with Group B antisera; the other does not.
Medium 培養(yǎng)基 ATCC? Medium 3: Nutrient agar or nutrient broth
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37℃
Atmosphere 需氧情況: Aerobic
Name of Depositor 寄存人 CDC
Chain of Custody 來源國家 CDC <-- University of Missouri
Isolation 分離基物 Tissue, animal - pools of heart and liver from 4-week-old chickens
References 參考文獻(xiàn) Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.
Judicial Commission of the Internat. The type species of the genus Salmonella Lignieres 1900 is Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with the type strain LT2T, and conservation of the epithet enterica in Salmonella enterica over all earlier epithets that may be applied to this species. Opinion 80. Int. J. Syst. Evol. Microbiol. 55: 519-520, 2005. PubMed: 15653929
Tindall BJ, et al. Nomenclature and taxonomy of the genus Salmonella. Int. J. Syst. Evol. Microbiol. 55: 521-524, 2005. PubMed: 15653930
Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1682, 2005
Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.
Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.
CDC, personal communication ColiGel Test. Charm. Pathogel? Test. Charm. U.S. Pharmacopeia General Chapters:Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. Rockville, MD USP34-NF29, 2011 ISO/CD 11133:2009,
Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions) Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.
Cross References
Nucleotide (GenBank) : AX109618 Sequence 351 from Patent WO0123604.
Nucleotide (GenBank) : AX111148 Sequence 1881 from Patent WO0123604.
Nucleotide (GenBank) : AX111476 Sequence 2209 from Patent WO0123604.
Nucleotide (GenBank) : U67869 Salmonella typhimurium SirA gene, complete cds.
Nucleotide (GenBank) : U06133 Salmonella typhimurium ATCC 14028 isolate MS1 Tn10 insertion site.
Nucleotide (GenBank) : U06126 Salmonella typhimurium ATCC 14028 isolate MS1321 Tn10 insertion site.
Nucleotide (GenBank) : U06127 Salmonella typhimurium ATCC 14028 isolate MS1413 Tn10 insertion site.
Nucleotide (GenBank) : U06128 Salmonella typhimurium ATCC 14028 isolate MS1523 Tn10 insertion site.
Nucleotide (GenBank) : U06129 Salmonella typhimurium ATCC 14028 isolate MS1521 Tn10 insertion site.
Nucleotide (GenBank) : U06130 Salmonella typhimurium ATCC 14028 isolate MS1633 Tn10 insertion site.
Nucleotide (GenBank) : U06131 Salmonella typhimurium ATCC 14028 isolate MS1668 Tn10 insertion site.
Nucleotide (GenBank) : U06132 Salmonella typhimurium ATCC 14028 isolate MS3493 Tn10 insertion site.
Nucleotide (GenBank) : U06134 Salmonella typhimurium ATCC 14028 isolate MS2097 Tn10 insertion site.
Nucleotide (GenBank) : U06135 Salmonella typhimurium ATCC 14028 isolate MS2101 Tn10 insertion site.
Nucleotide (GenBank) : U06136 Salmonella typhimurium ATCC 14028 isolate MS4290 Tn10 insertion site.
Nucleotide (GenBank) : U06137 Salmonella typhimurium ATCC 14028 isolate MS3747 Tn10 insertion site.
Nucleotide (GenBank) : U06138 Salmonella typhimurium ATCC 14028 isolate MS5076 Tn10 insertion site.
Nucleotide (GenBank) : U06139 Salmonella typhimurium ATCC 14028 isolate MS7481 Tn10 insertion site.
Nucleotide (GenBank) : U06140 Salmonella typhimurium ATCC 14028 isolate MS6290 Tn10 insertion site.
Nucleotide (GenBank) : U06141 Salmonella typhimurium ATCC 14028 isolate MS9020 Tn10 insertion site.
Nucleotide (GenBank) : U06142 Salmonella typhimurium ATCC 14028 isolate MS8282 Tn10 insertion site.
Nucleotide (GenBank) : U31849 Salmonella typhimurium pagD, envE, msgA and envF genes, complete cds.
Nucleotide (GenBank) : U65941 Salmonella typhimurium O-antigen acetylase (oafA) gene, complete cds.
Nucleotide (GenBank) : AF129435 Salmonella typhimurium bovine colonization factor BcfC gene, complete cds.
Nucleotide (GenBank) : AF130422 Salmonella typhimurium bovine colonization factor operon, complete sequence.
Nucleotide (GenBank) : AF175343 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch1c, genomic survey sequence
Nucleotide (GenBank) : AF175344 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch6c, genomic survey sequence
Nucleotide (GenBank) : AF175345 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch8c, genomic survey sequence
Nucleotide (GenBank) : AF175357 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh2, genomic survey sequence
Nucleotide (GenBank) : AF175358 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh3, genomic survey sequence
Nucleotide (GenBank) : AF175359 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh4, genomic survey sequence
Nucleotide (GenBank) : AF175360 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh6, genomic survey sequence
Nucleotide (GenBank) : AF175361 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh8, genomic survey sequence
Nucleotide (GenBank) : AF175362 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh9, genomic survey sequence
Nucleotide (GenBank) : AF175346 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch14c, genomic survey sequence
Nucleotide (GenBank) : AF175347 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch16c, genomic survey sequence
Nucleotide (GenBank) : AF175348 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch20c, genomic survey sequence
Nucleotide (GenBank) : AF175349 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch26c, genomic survey sequence
Nucleotide (GenBank) : AF175350 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch36c, genomic survey sequence
Nucleotide (GenBank) : AF175351 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch37c, genomic survey sequence
Nucleotide (GenBank) : AF175352 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch39c, genomic survey sequence
Nucleotide (GenBank) : AF175353 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch43c, genomic survey sequence
Nucleotide (GenBank) : AF175354 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch44c, genomic survey sequence
Nucleotide (GenBank) : AF175355 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch45c, genomic survey sequence
Nucleotide (GenBank) : AF175356 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch46c, genomic survey sequence
Nucleotide (GenBank) : AF175363 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh13, genomic survey sequence
Nucleotide (GenBank) : AF175364 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh14, genomic survey sequence
Nucleotide (GenBank) : AF175365 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh15, genomic survey sequence
Nucleotide (GenBank) : AF175366 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh16, genomic survey sequence
Nucleotide (GenBank) : AF175367 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh17, genomic survey sequence
Nucleotide (GenBank) : AF175368 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh19, genomic survey sequence
Nucleotide (GenBank) : AF175369 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh22, genomic survey sequence
Nucleotide (GenBank) : AF175370 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh25, genomic survey sequence
Nucleotide (GenBank) : AF175371 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh26, genomic survey sequence
Nucleotide (GenBank) : AF175372 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh27, genomic survey sequence
Nucleotide (GenBank) : AF175373 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh29, genomic survey sequence
Nucleotide (GenBank) : AF175374 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh30, genomic survey sequence
Nucleotide (GenBank) : AF175375 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh31, genomic survey sequence
Nucleotide (GenBank) : AF175376 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh32, genomic survey sequence
Nucleotide (GenBank) : AF175377 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh34, genomic survey sequence
Nucleotide (GenBank) : AF175378 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh36, genomic survey sequence
Nucleotide (GenBank) : AF175379 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh37, genomic survey sequence
Nucleotide (GenBank) : AF175380 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh38, genomic survey sequence
Nucleotide (GenBank) : AF175381 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh39, genomic survey sequence
Nucleotide (GenBank) : AF175382 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh41, genomic survey sequence
Nucleotide (GenBank) : AF175383 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh45, genomic survey sequence
Nucleotide (GenBank) : AF175384 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh50, genomic survey sequence
Nucleotide (GenBank) : U18559 Salmonella typhimurium long polar fimbriae proteins (lpfA, lpfB, lpfC, lpfD, lpfE) genes, complete cds and (orf1, orf2) genes, partial cds.
Nucleotide (GenBank) : U21676 Salmonella typhimurium PrgH protein (prgH), PrgI protein (prgI), PrgJ protein (prgJ), and PrgK protein (prgK) genes complete cds, and OrgA protein (orgA) partial cds.
ATCC 14028 統(tǒng)一編號(hào)
Strain Designations 菌株別名 : CDC 6516-60 /
Type Strain 模式菌株 : no /
Biosafety Level 生物安全等級(jí) : 2
Deposited As Salmonella typhimurium (Loeffler) Castellani and Chalmers
Application 用途 Control Culture Media testing
Preparatory test control
Enteric Research
Emerging infectious disease research
Pharmaceutical and Personal Care
Water testing
Isolation 分離基物 Tissue, animal - pools of heart and liver from 4-week-old chickens
Type Strain 模式菌株 no
Biosafety Level 生物安全等級(jí) 2
Antigenic Properties I 4,5,12:i:1,2 (Determined by depositor)
Product Format 提供形式 freeze-dried
Storage Conditions 保藏方法
Frozen（冷凍物）: -80℃ or colder
Freeze-Dried（凍干物）: 2℃ to 8℃
Live Culture（活物）: See Propagation Section
Preceptrol? yes
Type Strain 模式菌株 no
Antigenic Properties I 4,5,12:i:1,2 (Determined by depositor)
Comments 注釋 This strain is recommended by ATCC for use in the tests described in U.S. Pharmacopeia, 23rd rev.,, p. 2180, 1995, where only the taxon is specified. The depositor states that this strain possesses these antigenic properties: 4,5,12:i:1,2. Two colony types can be distinguished on sheep blood agar. One colony type reacts with Group B antisera; the other does not.
Medium 培養(yǎng)基 ATCC? Medium 3: Nutrient agar or nutrient broth
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37℃
Atmosphere 需氧情況: Aerobic
Name of Depositor 寄存人 CDC
Chain of Custody 來源國家 CDC <-- University of Missouri
Isolation 分離基物 Tissue, animal - pools of heart and liver from 4-week-old chickens
References 參考文獻(xiàn) Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.
Judicial Commission of the Internat. The type species of the genus Salmonella Lignieres 1900 is Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with the type strain LT2T, and conservation of the epithet enterica in Salmonella enterica over all earlier epithets that may be applied to this species. Opinion 80. Int. J. Syst. Evol. Microbiol. 55: 519-520, 2005. PubMed: 15653929
Tindall BJ, et al. Nomenclature and taxonomy of the genus Salmonella. Int. J. Syst. Evol. Microbiol. 55: 521-524, 2005. PubMed: 15653930
Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1682, 2005
Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.
Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.
CDC, personal communication ColiGel Test. Charm. Pathogel? Test. Charm. U.S. Pharmacopeia General Chapters:Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. Rockville, MD USP34-NF29, 2011 ISO/CD 11133:2009,
Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions) Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.
Cross References
Nucleotide (GenBank) : AX109618 Sequence 351 from Patent WO0123604.
Nucleotide (GenBank) : AX111148 Sequence 1881 from Patent WO0123604.
Nucleotide (GenBank) : AX111476 Sequence 2209 from Patent WO0123604.
Nucleotide (GenBank) : U67869 Salmonella typhimurium SirA gene, complete cds.
Nucleotide (GenBank) : U06133 Salmonella typhimurium ATCC 14028 isolate MS1 Tn10 insertion site.
Nucleotide (GenBank) : U06126 Salmonella typhimurium ATCC 14028 isolate MS1321 Tn10 insertion site.
Nucleotide (GenBank) : U06127 Salmonella typhimurium ATCC 14028 isolate MS1413 Tn10 insertion site.
Nucleotide (GenBank) : U06128 Salmonella typhimurium ATCC 14028 isolate MS1523 Tn10 insertion site.
Nucleotide (GenBank) : U06129 Salmonella typhimurium ATCC 14028 isolate MS1521 Tn10 insertion site.
Nucleotide (GenBank) : U06130 Salmonella typhimurium ATCC 14028 isolate MS1633 Tn10 insertion site.
Nucleotide (GenBank) : U06131 Salmonella typhimurium ATCC 14028 isolate MS1668 Tn10 insertion site.
Nucleotide (GenBank) : U06132 Salmonella typhimurium ATCC 14028 isolate MS3493 Tn10 insertion site.
Nucleotide (GenBank) : U06134 Salmonella typhimurium ATCC 14028 isolate MS2097 Tn10 insertion site.
Nucleotide (GenBank) : U06135 Salmonella typhimurium ATCC 14028 isolate MS2101 Tn10 insertion site.
Nucleotide (GenBank) : U06136 Salmonella typhimurium ATCC 14028 isolate MS4290 Tn10 insertion site.
Nucleotide (GenBank) : U06137 Salmonella typhimurium ATCC 14028 isolate MS3747 Tn10 insertion site.
Nucleotide (GenBank) : U06138 Salmonella typhimurium ATCC 14028 isolate MS5076 Tn10 insertion site.
Nucleotide (GenBank) : U06139 Salmonella typhimurium ATCC 14028 isolate MS7481 Tn10 insertion site.
Nucleotide (GenBank) : U06140 Salmonella typhimurium ATCC 14028 isolate MS6290 Tn10 insertion site.
Nucleotide (GenBank) : U06141 Salmonella typhimurium ATCC 14028 isolate MS9020 Tn10 insertion site.
Nucleotide (GenBank) : U06142 Salmonella typhimurium ATCC 14028 isolate MS8282 Tn10 insertion site.
Nucleotide (GenBank) : U31849 Salmonella typhimurium pagD, envE, msgA and envF genes, complete cds.
Nucleotide (GenBank) : U65941 Salmonella typhimurium O-antigen acetylase (oafA) gene, complete cds.
Nucleotide (GenBank) : AF129435 Salmonella typhimurium bovine colonization factor BcfC gene, complete cds.
Nucleotide (GenBank) : AF130422 Salmonella typhimurium bovine colonization factor operon, complete sequence.
Nucleotide (GenBank) : AF175343 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch1c, genomic survey sequence
Nucleotide (GenBank) : AF175344 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch6c, genomic survey sequence
Nucleotide (GenBank) : AF175345 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch8c, genomic survey sequence
Nucleotide (GenBank) : AF175357 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh2, genomic survey sequence
Nucleotide (GenBank) : AF175358 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh3, genomic survey sequence
Nucleotide (GenBank) : AF175359 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh4, genomic survey sequence
Nucleotide (GenBank) : AF175360 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh6, genomic survey sequence
Nucleotide (GenBank) : AF175361 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh8, genomic survey sequence
Nucleotide (GenBank) : AF175362 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh9, genomic survey sequence
Nucleotide (GenBank) : AF175346 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch14c, genomic survey sequence
Nucleotide (GenBank) : AF175347 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch16c, genomic survey sequence
Nucleotide (GenBank) : AF175348 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch20c, genomic survey sequence
Nucleotide (GenBank) : AF175349 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch26c, genomic survey sequence
Nucleotide (GenBank) : AF175350 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch36c, genomic survey sequence
Nucleotide (GenBank) : AF175351 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch37c, genomic survey sequence
Nucleotide (GenBank) : AF175352 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch39c, genomic survey sequence
Nucleotide (GenBank) : AF175353 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch43c, genomic survey sequence
Nucleotide (GenBank) : AF175354 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch44c, genomic survey sequence
Nucleotide (GenBank) : AF175355 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch45c, genomic survey sequence
Nucleotide (GenBank) : AF175356 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone ch46c, genomic survey sequence
Nucleotide (GenBank) : AF175363 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh13, genomic survey sequence
Nucleotide (GenBank) : AF175364 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh14, genomic survey sequence
Nucleotide (GenBank) : AF175365 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh15, genomic survey sequence
Nucleotide (GenBank) : AF175366 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh16, genomic survey sequence
Nucleotide (GenBank) : AF175367 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh17, genomic survey sequence
Nucleotide (GenBank) : AF175368 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh19, genomic survey sequence
Nucleotide (GenBank) : AF175369 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh22, genomic survey sequence
Nucleotide (GenBank) : AF175370 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh25, genomic survey sequence
Nucleotide (GenBank) : AF175371 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh26, genomic survey sequence
Nucleotide (GenBank) : AF175372 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh27, genomic survey sequence
Nucleotide (GenBank) : AF175373 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh29, genomic survey sequence
Nucleotide (GenBank) : AF175374 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh30, genomic survey sequence
Nucleotide (GenBank) : AF175375 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh31, genomic survey sequence
Nucleotide (GenBank) : AF175376 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh32, genomic survey sequence
Nucleotide (GenBank) : AF175377 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh34, genomic survey sequence
Nucleotide (GenBank) : AF175378 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh36, genomic survey sequence
Nucleotide (GenBank) : AF175379 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh37, genomic survey sequence
Nucleotide (GenBank) : AF175380 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh38, genomic survey sequence
Nucleotide (GenBank) : AF175381 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh39, genomic survey sequence
Nucleotide (GenBank) : AF175382 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh41, genomic survey sequence
Nucleotide (GenBank) : AF175383 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh45, genomic survey sequence
Nucleotide (GenBank) : AF175384 Salmonella typhimurium ATCC14028 Salmonella typhimurium genomic clone cjh50, genomic survey sequence
Nucleotide (GenBank) : U18559 Salmonella typhimurium long polar fimbriae proteins (lpfA, lpfB, lpfC, lpfD, lpfE) genes, complete cds and (orf1, orf2) genes, partial cds.
Nucleotide (GenBank) : U21676 Salmonella typhimurium PrgH protein (prgH), PrgI protein (prgI), PrgJ protein (prgJ), and PrgK protein (prgK) genes complete cds, and OrgA protein (orgA) partial cds.
常見問題
問：細(xì)胞的運(yùn)輸方式有哪些？有什么區(qū)別？
答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。
問：為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣？
答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！
問：培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎？
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。
問：培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么？
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
問：剛買回來的細(xì)胞如何凍存留種呢？
答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。
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