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      產(chǎn)品詳情

      Preceptrol? Culture

      Organism:

      Escherichia coli MigulaCaslani and Chalmers

      Designations:

      FDA strain Seattle 1946 [DSM 1103, NCIB 12210]

      Isolation:

      clinical isolate

      Depositor:

      FDA

      History:

      ATCC <<--FDA<<--F. Schoenknecht

      Biosafety Level:

      1

      Shipped:

      freeze-dried

      Growth Conditions:

      ATCC medium18: Trypticase soy agar
      Temperature: 37.0°C
      Atmosphere: Aerobic

      Permits/Forms:

      In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

      Cross References:

      Nucleotide GenBank: AF038431 Escherichia coli DNA gyrase A gyrAgene, partial cds.

      Nucleotide GenBank: AX110212 Sequence 945 from Patent WO0123604.

      Nucleotide GenBank: AX110242 Sequence 975 from Patent WO0123604.

      Nucleotide GenBank: AX111547 Sequence 2280 from Patent WO0123604.

      Nucleotide GenBank: X80724 E. coli ATCC 25922gene for 16S rRNA.

      Comments:

      Incorrectly cited in previous editions of this catalog as identical to NCTC 10418

      Applications:

      Control Culture [92260] [92376] [92377]

      control strain [21528] [21531] [92352]

      detection of [92254] [92282] [92381] [92834]

      evaluation of Mueller-Hinton agar [21524] [21585]

      media testing [21529] [92345] [92390] [92429] [92819] [92844] [92845]

      quality control [92216]

      quality control strain [92099] [92126] [92135] [92137] [92146] [92147] [92150] [92153] [92159] [92160] [92163]

      susceptibility disc testing [21523] [92224] [92354] [92355]

      susceptibility disc testing cephalexin [45872]

      susceptibility disc testing cephaloglycin [45872]

      susceptibility disc testing cephaloridine cephalomycin [21533] [45872]

      susceptibility disc testing cephalothin [45872]

      susceptibility disc testing chloramphenicol [58588]

      susceptibility disc testing colistin colimycin [58588]

      susceptibility disc testing gentamicins gentamicin [58588]

      susceptibility disc testing kanamycin [58588]

      susceptibility disc testing nalidixic acid [58588]

      susceptibility disc testing neomycin [58588]

      susceptibility disc testing tetracycline [58588]

      susceptibility testing [21525] [21527] [21533] [90283] [92353] [92422] [92423] [92424] [92425] [92788]

      testing [92374]

      quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, Biosynth, Difco, IDS, Micro-Media, MicroScan [Reg TM], Roche Diagnostics, and Sensititre products

      Related Products:

      also available as SafeTsource?:ATCC 25922NA

      purified DNA: ATCC 25922D-5

      References:

      21523: Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

      21524: Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

      21525: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

      21527: Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M11-A7.

      21528: Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A.

      21529: Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

      21531: Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M26-A.

      21533: Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

      21560: U.S. Food & Drug AdministrationEnterotoxigenic Escherichia coli: Heat-stabile enterotoxin human, heat-stabile enterotoxin porcine, and heat-labile enterotoxin

      In: U.S. Food & Drug AdministrationBacteriological analytical manual8th ed.Gaithersburg, MDAOAC Internationalpp. 24.12-24.14, 1995

      21585: US General Services Administration Mueller Hinton Medium, dehydrated. Washington, DC:US General Services Administration;Commercial Item Description A-A-50904A, 1986

      27379: McNamara BP, Wolfe AJ. Coexpression of the long and short forms of CheA, the chemotaxis histidine kinase, by members of the family Enterobacteriaceae. J. Bacteriol. 179: 1813-1818, 1997. PubMed: 9045846

      32539: Nagar-Anthal KR, et al. The pterin lumazine inhibits growth of methanogens and methane formation. Arch. Microbiol. 166: 136-140, 1996.

      32591: Wang RF, et al. PCR detection and quantitiation of predominant anaerobic bacteria in human and animal fecal samples. Appl. Environ. Microbiol. 62: 1242-1247, 1996. PubMed: 8919784

      33008: Blondelle SE, et al. Identification of inhibitors of melittin using nonsupport-bound combinatorial libraries. J. Biol. Chem. 271: 4093-4099, 1996. PubMed: 8626746

      45872: . . Literature issued December 20, 1970 on Cephalothin Discs by Eli Lilly and Co., Indianapolis, IN. : .

      58588: Boyle VJ, et al. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3: 418-424, 1973. PubMed: 4790600

      90283: Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

      92099: API 20E QC Set. bioMerieux.

      92126: BD Crystal E/NF QC Set. BD Becton Dickinson; Crystal

      92135: BD Enterotube II QC Set. BD Becton Dickinson; Enterotube

      92137: BD Phoenix Gram Negative NMIC and NMIC/ID QC Set. BD Becton Dickinson; Phoenix

      92146: Colilert. IDEXX.

      92147: Colisure. IDEXX.

      92150: MicroScan Dried Gram Negative Panel QC Set. Dade/Siemens.

      92153: MicroScan Rapid Gram Negative QC Set. Dade/Siemens.

      92159: Sensititre Automated Gram Positive ID. Trek Diagnostic Systems.

      92160: Sensititre MIC Susceptibility. Trek Diagnostic Systems.

      92163: Sensititre Aquaculture MIC Susceptibility. Trek Diagnostic Systems.

      92216: Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A.

      92222: Abbreviated Identification of Bacteria and Yeast; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M35-A2.

      92224: Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A.

      92254: Enumeration of Escherichia coli and the Coliform Bacteria: LST-MUG Method for Detecting E.coli in chilled or Frozen Foods Exclusive of Bivalve Mollucan Sellfish. Washington, DC:US Food & Drug Administration;Bacteriological Analytical Manual Online Chapt. 4.II.3, 2002

      92260: Identification of Foodborne Bacterial Pathogens by Gene Probes: Enterotoxigenic Escherichia coli: Heat-Stable Enterotoxin Human, Heat-Stable Enterotoxin Porcine, and Heat-Labile Enterotoxin. Washington, DC:US Food & Drug Administration;Bacteriological Analytical Manual Online Chapt. 24, 2001

      92282: Salmonella in Foods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 991.38.

      92345: Food microbiology. Method 12.3: Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of coagulase-positive staphylococci Staphyloccus aureus and other species--Detection and MPN technique for low numbers. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 5013.12.3:2004.

      92352: Procedures for the Recovery and Identification of Parasites from the Intestinal Tract: Approved Guideline - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M28-A2.

      92353: Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P.

      92354: Methods for Antimicrobial Dilution and Disk susceptibility Testing of Infrequently Isolated or Fastidious Bacteria: Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M45-A.

      92355: Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A.

      92374: Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin ADA-V. Washington, DC:Environmental Protection Agency;EPA EPA Method 1605, 2001

      92376: Fecal Coliforms in Sewage Sludge Biosolidsby Multiple-Tube Fermenatation using A-1 medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1681, 2005

      92377: Salmonella in Sewage Sludge Biosolidsby Modified Semisolid Rappaport-Vassiliadis MSRVMedium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1682, 2005

      92381: Microbiology of food and animal feeding stuffs-- Horizontal method for the enumeration of coagulase-positive staphylococci Staphylococcus aureus and other species--Part3: Detection and MPN technique for low numbers. Geneva Switzerland:International Organization for Standardization/ANSI;ISO ISO 6888-3:2003.

      92390: Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva Switzerland:International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

      92422: Medical microbiology; methods for the determination of susceptibility of pathogens except mycobacteriato antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-3: 1989

      92423: Medical microbiology -- Methods for the determination of susceptibility of pathogens except mycobacteriato antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration MBCwith the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-7: 1994, 1994

      92424: Medical microbiology: methods for the determination of susceptibility of pathogens except mycobacteriato antimicrobial agents; determination of MICs by agar dilution method. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-6: 1989, 1989

      92425: Medical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002

      92429: Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003

      92430: Medical microbiology--Culture media -- Part 4: Transport systems for specimens containing bacteria. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-4: 2003, 2003

      92788: Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006.

      92819: Microbiology of food and animal feeding stuffs --- Horizontal method for the detection and enumeration of Listeria monocytogenes --- Part 1: Detection method, Annex B [BS 5763-18:1997]. London, UK:British Standards Institution;British Standard BS EN ISO 11290-1:1997.

      92834: Microbiology of food and animal feeding stuffs --- Horizontal method for the determination of low numbers of presumptive Bacillus cereus --- Most probable number technique and detection method. London, UK:British Standards Institution;British Standard BS EN ISO 21871:2006.

      92844: Microbiology of food and animal feeding stuffs --- Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli -- Part 3: Most probable number techniques using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide. London, UK:British Standards Institution;British Standard DD ISO/TS 16649-3:2005.

      92845: Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

      16173638: Colilert-18 QC Set. IDEXX.

      16173653: VITEK 2 AST-GN QC Set. bioMerieux.

      16173660: RapID ONE QC Set. Remel.

      16173666: RapID SS/u QC Set. Remel.

      16173670: MicroScan ESBL plus QC Set. Dade/Siemens.

      16173672: MicroScan Rapid Gram Negative ID QC Set. Dade/Siemens.

      16173673: MicroScan Rapid Neg ID Type 4 QC Set. Dade/Siemens.

      16173674: MicroScan Streamlined ID QC Set. Dade/Siemens.

      16173675: MicroScan Synergies plus Gram Negative QC Set. Dade/Siemens.

      16173690: Colitag QC Set

      16173836: ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions

      16173837: Corry, JEL, Curtis, GDW and Baird, RM EdsHandbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

      常見問題
      問:細(xì)胞的運(yùn)輸方式有哪些?有什么區(qū)別?

      答:公司提供兩種運(yùn)輸方式供老師選擇,1、復(fù)蘇的活細(xì)胞:采用常溫發(fā)貨的方式,收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟,細(xì)胞成活率較高。2、凍存的細(xì)胞:采用干冰運(yùn)輸,一般情況下發(fā)貨是2支凍存管,收到后放-80過夜,第二天轉(zhuǎn)入液氮長期存儲(chǔ),擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快,一般一兩天即可收到,缺點(diǎn)是需要自己復(fù)蘇。

      問:為什么你們的細(xì)胞和其他公司的細(xì)胞培養(yǎng)條件不一樣?

      答:我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息,如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案,根據(jù)客戶的意愿進(jìn)行選擇!

      問:培養(yǎng)瓶的培養(yǎng)液可以重復(fù)使用嗎?

      答:不可以重復(fù)使用,一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出,充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多,通常在3-5%,以維持細(xì)胞存活,控制生長速度,不可以用來做細(xì)胞培養(yǎng)使用。

      問:培養(yǎng)細(xì)胞在鏡下觀察發(fā)現(xiàn)有一些白色的圓點(diǎn)是什么?

      答:細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn),一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物,懸浮細(xì)胞也會(huì)有這種現(xiàn)象,出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常,白色的圓點(diǎn)是分散分布的,聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑,技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。

      問:剛買回來的細(xì)胞如何凍存留種呢?

      答:一般情況下,我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種,可先凍存1-2支凍存管,凍存的細(xì)胞數(shù)量多一些,便于后期復(fù)蘇。購買原代細(xì)胞的客戶,要充分考慮該細(xì)胞的傳代次數(shù)限制,人源原代細(xì)胞大概可以傳7代左右,鼠源的可以傳3代左右,對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞,不建議凍存,收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。

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