一鍵復(fù)制產(chǎn)品信息
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- 電話：400-1016-218
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- 地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國家大學(xué)科技園A401室
產(chǎn)品詳情
Acinetobacter venetianus Vaneechoutte et al. ex Di Cello et al. (ATCC?31012?)
|
Deposited As |
Arthrobacter sp. |
|
Strain Designations |
RAG-1 |
|
Application |
Degrades petroleum crude oil Produces apoemulsans Produces emulsans Produces emulsifier EF-RAG Produces esterase Production of exocellular esterase |
|
Isolation |
Tar on beach |
|
Type Strain |
no |
|
Biosafety Level |
1 |
|
Product Format |
freeze-dried |
|
Preceptrol? |
no |
|
Type Strain |
no |
|
Comments |
Fimbriae Enantioselective reduction of acetyldimethylphenylsilane |
|
Medium |
ATCC? Medium 3: Nutrient agar or nutrient broth |
|
Growth Conditions |
Temperature: 30.0°C |
|
Name of Depositor |
Emulsan Biotechnologies Inc. |
|
Isolation |
Tar on beach ATCC 31012菌種， 威尼斯不動(dòng)桿菌 |
|
References |
Shabtai Y, Gutnick DL. Exocellular esterase and emulsan release from the cell surface of Acinetobacter calcoaceticus. J. Bacteriol. 161: 1176-1181, 1985. PubMed: 3838301 Rosenberg E, et al. Emulsifier of Arthrobacter RAG-1: isolation and emulsifying properties. Appl. Environ. Microbiol. 37: 402-408, 1979. PubMed: 36840 Rosenberg E, et al. Emulsifier of Arthrobacter RAG-1: specificity of hydrocarbon substrate. Appl. Environ. Microbiol. 37: 409-413, 1979. PubMed: 453821 Zuckerberg A, et al. Emulsifier of Arthrobacter RAG-1: chemical and physical properties. Appl. Environ. Microbiol. 37: 414-420, 1979. PubMed: 453822 Reisfeld A, et al. Microbial degradation of crude oil: Factors affecting the dispersion in sea water by mixed and pure cultures. Appl. Microbiol. 24: 363-368, 1972. PubMed: 4562475 Horowitz A, et al. Sequential growth of bacteria on crude oil. Appl. Microbiol. 30: 10-19, 1975. PubMed: 1096820 Gutnick D, Rosenberg AE. Cleaning of cargo compartments. US Patent 3,941,692 dated Mar 2 1976 Gutnick DL, Rosenberg E. Production of alpha-emulsans. US Patent 4,230,801 dated Oct 28 1980 Rosenberg E, et al. Production of alpha-emulsans. US Patent 4,234,689 dated Nov 18 1980 Gutnick DL, et al. alpha-Emulsans. US Patent 4,395,354 dated Jul 26 1983 Hayes ME. Personal care products containing bioemulsifiers. US Patent 4,999,195 dated Mar 12 1991 . . Appl. Environ. Microbiol. 44: 929-937, 1982. Syldatk C, et al. Enantioselective reduction of acetyldimethylphenylsilane: a screening with thirty strains of microorganisms. Biotechnol. Lett. 10: 731-736, 1988. |
|
Cross References |
Nucleotide (GenBank) : AJ295007 Acinetobacter venetianus ucleotide (GenBank) : D73425 Acinetobacter sp. gene for DNA gyrase subunit B protein, C terminal side, partial cds. NsNucleotide (GenBank) : D73440 Acinetobacter sp. gene for Dtrain ATCC 31012. Nucleotide (GenBank) : AB008696 Acinetobacter sp. DNA for DNA gyrase subunit B, partial cds, strain ATCC 31012. Nide, partial cdA gyrase subunit B protein, N terminal s16S rRNA gene, s. |
常見問題
答：公司提供兩種運(yùn)輸方式供老師選擇，1、復(fù)蘇的活細(xì)胞：采用常溫發(fā)貨的方式，收到即可觀察密度并判斷是否進(jìn)行傳代操作。優(yōu)勢(shì)是省去復(fù)蘇的步驟，細(xì)胞成活率較高。2、凍存的細(xì)胞：采用干冰運(yùn)輸，一般情況下發(fā)貨是2支凍存管，收到后放-80過夜，第二天轉(zhuǎn)入液氮長期存儲(chǔ)，擇機(jī)復(fù)蘇。優(yōu)勢(shì)是發(fā)貨快，一般一兩天即可收到，缺點(diǎn)是需要自己復(fù)蘇。
答：我公司提供的細(xì)胞大部分都參考資源庫的培養(yǎng)信息，如ATCC、DSMZ、中科院等等官方平臺(tái)。也有少部分細(xì)胞為客戶提供了替代培養(yǎng)方案，根據(jù)客戶的意愿進(jìn)行選擇！
答：不可以重復(fù)使用，一般從我公司發(fā)出的細(xì)胞都需要達(dá)到一定的密度后發(fā)出，充液的培養(yǎng)基血清比例會(huì)比正常培養(yǎng)時(shí)所用到的培養(yǎng)液低很多，通常在3-5%，以維持細(xì)胞存活，控制生長速度，不可以用來做細(xì)胞培養(yǎng)使用。
答：細(xì)胞在鏡下發(fā)現(xiàn)圓形的白色的點(diǎn)點(diǎn)，一般情況下是為貼壁的細(xì)胞或脫落的細(xì)胞死亡后的產(chǎn)物，懸浮細(xì)胞也會(huì)有這種現(xiàn)象，出現(xiàn)圓形的光圈一樣的圓點(diǎn)。通常，白色的圓點(diǎn)是分散分布的，聚團(tuán)類的懸浮細(xì)胞可能會(huì)聚團(tuán)出現(xiàn)白色的亮斑，技術(shù)老師可以繼續(xù)培養(yǎng)并觀察。
答：一般情況下，我公司建議客戶收到細(xì)胞后傳1-2代后即可安排凍存留種，可先凍存1-2支凍存管，凍存的細(xì)胞數(shù)量多一些，便于后期復(fù)蘇。購買原代細(xì)胞的客戶，要充分考慮該細(xì)胞的傳代次數(shù)限制，人源原代細(xì)胞大概可以傳7代左右，鼠源的可以傳3代左右，對(duì)于一些能傳代次數(shù)很少的原代細(xì)胞，不建議凍存，收到后調(diào)整狀態(tài)后即可安排實(shí)驗(yàn)。
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